CAJ | 학술논문

采用不同溶剂分级提取骆驼蓬(Peganum harmala L.)根、茎、叶及种子中的蛋白质,结果表明总蛋白以种子中的蛋白含量最高,而营养器官以叶中的蛋白含量较高.当年种子总蛋白含量显著高于贮藏种子.不同溶剂分级提取的当年生种子、根、茎和叶中各组分,以碱提组分蛋白含量最高.用含不同盐离子浓度的缓冲液和不同pH值的广泛缓冲液提取骆驼蓬种子的蛋白质,结果表明优化后的缓冲液条件为含0.2 mol/L NaCl,pH=7.0～8.0的5 mmol/LPBS缓冲液.硫酸铵沉淀法获得的骆驼蓬种子中的蛋白粗提物(1.2 mg/mL)对供试真菌交格链孢菌(Ahernaria alternata)、指状青霉菌(Penicillium degitatum)、灰霉病菌(Botrytis cinerea)、稻瘟病菌(Magnaporthe grisea)和意大利青霉菌(Penicillium italicum)等5种植物病原真菌均有抑菌作用,其中对意大利青霉菌和交格链孢菌表现出较好的抑菌活性,抑菌环直径分别为19.50和18.50 mm.对供试细菌表皮葡萄球菌(Staphylococcus epidermidis)、粪肠球菌(Enterococcus faecalis)、臭鼻克雷伯菌(Klebsiella penumoniae)、福氏志贺氏菌(Shigella flexneri)、金黄色葡萄球菌(Staphylococcus altreus)和鲍曼不动杆菌(Acinetobacter baumannii)等6种病原细菌也有抑菌作用,其中对臭鼻克雷伯菌、福氏志贺氏菌、表皮葡萄球菌等病原细菌等有较好的抑制作用,抑菌圈直径分别10.20、10.10和9.30 mm.
채용불동용제분급제취락타봉(Peganum harmala L.)근、경、협급충자중적단백질,결과표명총단백이충자중적단백함량최고,이영양기관이협중적단백함량교고.당년충자총단백함량현저고우저장충자.불동용제분급제취적당년생충자、근、경화협중각조분,이감제조분단백함량최고.용함불동염리자농도적완충액화불동pH치적엄범완충액제취락타봉충자적단백질,결과표명우화후적완충액조건위함0.2 mol/L NaCl,pH=7.0～8.0적5 mmol/LPBS완충액.류산안침정법획득적락타봉충자중적단백조제물(1.2 mg/mL)대공시진균교격련포균(Ahernaria alternata)、지상청매균(Penicillium degitatum)、회매병균(Botrytis cinerea)、도온병균(Magnaporthe grisea)화의대리청매균(Penicillium italicum)등5충식물병원진균균유억균작용,기중대의대리청매균화교격련포균표현출교호적억균활성,억균배직경분별위19.50화18.50 mm.대공시세균표피포도구균(Staphylococcus epidermidis)、분장구균(Enterococcus faecalis)、취비극뢰백균(Klebsiella penumoniae)、복씨지하씨균(Shigella flexneri)、금황색포도구균(Staphylococcus altreus)화포만불동간균(Acinetobacter baumannii)등6충병원세균야유억균작용,기중대취비극뢰백균、복씨지하씨균、표피포도구균등병원세균등유교호적억제작용,억균권직경분별10.20、10.10화9.30 mm.
The proteins were extracted from the roots,stems,leaves and seeds of Peganum harmala L. By using the different solvents. The results showed that the seeds had the highest content of total protein, while the protein of vegetative organs was mainly distributed in the leaves. The protein content of current growth seeds was significantly higher than that of seeds stored. Among the proteins extracted from the different solvents,the components of total proteins got by NaOH extracting method is the highest percent. The optimized buffer conditions of the protein extraction under the different NaCl concentrations and the different pH buffers were 0.2 mol/L NaCl,pH =7.0-8.0 respectively. The determination of antimicrobial spectrum with the crude protein obtained by sulfate ammonium fractional precipitation indicated that the bacte-riostatic action were obvious to both 5 kinds of plant pathogenic fungi such as Altemaria alternate, Penicillium degita-tum,Botrytis cinerea,Magnaporthe grisea and Penicillium italicum,and 6 kinds of pathogenic bacteria such as Staphylo-coccus epidermidis,Enterococcus faecalis,Klebsiella penumoniae,Shigella flexneri,Staphylococcus aureus and Acinetobacter baumannii, especially have a distinct antimicrobial activity to two types of fungi, Penicillium degitatum and Altemaria al-ternate,with the diameter of inhibition ring was 19.50 and 18.50 mm respectively,and to three types of bacteria,Kleb-siella penumoniae, Shigella flexneri and Staphylococcus epidermidis, with the diameter of inhibition ring was 10.20,10.10 and 9.30 mm respectively.