CAJ | 학술논문

以6个桃品种的下胚轴为外植体,研究不同基因型、不同基本培养基及植物生长调节物质组合等对桃下胚轴离体再生不定芽的影响.结果显示:不同基因型桃下胚轴再生率差异显著;QL为'97-8-4'和'甜桃王'的最适基本培养基,MS为'金童5号'的最适基本培养基;植物生长调节物质用2.0 mg/L BA+0.04 mg/L NAA或单独使用2.0 mg/L BA的再生效果好.'97-8-4'在QL+2.0 mg/L BA+0.04 mg/L NAA+100 mg/L肌醇的培养基中再生率最高,为94.44%;'甜桃王' 在QL+2.0 mg/L BA+0.04 mg/L NAA+100 mg/L肌醇+500 mg/L CH的培养基中再生率最高,为80.42%;'金童5号'在QL+2.0 mg/L BA+0.04 mg/L NAA+200 mg/L肌醇的培养基中再生率最高,为78.00%.
이6개도품충적하배축위외식체,연구불동기인형、불동기본배양기급식물생장조절물질조합등대도하배축리체재생불정아적영향.결과현시:불동기인형도하배축재생솔차이현저;QL위'97-8-4'화'첨도왕'적최괄기본배양기,MS위'금동5호'적최괄기본배양기;식물생장조절물질용2.0 mg/L BA+0.04 mg/L NAA혹단독사용2.0 mg/L BA적재생효과호.'97-8-4'재QL+2.0 mg/L BA+0.04 mg/L NAA+100 mg/L기순적배양기중재생솔최고,위94.44%;'첨도왕' 재QL+2.0 mg/L BA+0.04 mg/L NAA+100 mg/L기순+500 mg/L CH적배양기중재생솔최고,위80.42%;'금동5호'재QL+2.0 mg/L BA+0.04 mg/L NAA+200 mg/L기순적배양기중재생솔최고,위78.00%.
Factors affecting shoot regeneration from hypocotyl explant in vitro including different genotypes,basic medium and plant growth regulators were investigated in peach.The results indicated that shoot regeneration rate from hypocotyl explant showed significant difference in different genotye.QL was the optimum basic medium for '97-8-4' and 'Tiantaowang'.MS was the optimum basic medium for 'Jintong 5'.The optimum plant gorwth regulators were 2.0 mg/L BA or the combination with 0.04 mg/L NAA.'97-8-4','Tiantaowang' and 'Jintong 5' showed the highest regeneration rate on QL+2.0 mg/L BA+0.04 mg/L NAA+100 mg/L inositol,QL+2.0 mg/L BA+0.04 mg/L NAA+100 mg/L inositol+500 mg/L CH,MS+2.0 mg/L BA+0.04 mg/L NAA+200 mg/L inositol respectively and the corresponding highest regeneration rate was 94.44%,80.42% and 78.00%,respectively.