华中科技大学学报（医学版）
華中科技大學學報（醫學版）
화중과기대학학보（의학판）
ACTA UNIVERSITATIS MEDICINAE TONGJI
2010年
1期
37-41,46
,共6页
姜黄素%鱼藤酮%PC12细胞%超氧化物歧化酶%活性氧类物质
薑黃素%魚籐酮%PC12細胞%超氧化物歧化酶%活性氧類物質
강황소%어등동%PC12세포%초양화물기화매%활성양류물질
curcumin%rotenone%PC12 cells%superoxide dismutase%reactive oxygen species
目的 探讨姜黄素(Cur)对鱼藤酮(Ro)致PC12细胞损伤的保护作用及其机制.方法 用鱼藤酮建立PC12细胞损伤模型;用四氮唑盐(MTT)比色法检测细胞增殖活力;苏木精-伊红染色观察细胞形态学变化;比色法检测细胞内总超氧化物歧化酶(SOD)的活性;DCFH-DA染色检测细胞内活性氧类物质(ROS)水平;流式细胞术(Annexin Ⅴ-FITC/PI双染法)检测PC12细胞的凋亡.结果 0.5 μmol/L姜黄素和1.0 μmol/L姜黄素均可减轻0.1 μmol/L鱼藤酮对PC12 细胞增殖活力的抑制,与鱼藤酮组比较差异有统计学意义(P＜0.01);明显减轻了细胞损伤的形态学改变;明显增加了 PC12 细胞内SOD的活性,与鱼藤酮组比较差异有统计学意义(P＜0.05);明显降低了PC12细胞内ROS的含量,明显抑制了鱼藤酮对PC12 细胞凋亡的诱导作用,与鱼藤酮组比较差异均有统计学意义(均P＜0.01).结论 姜黄素可拮抗鱼藤酮致PC12细胞的损伤,其机制可能与清除细胞内ROS,诱导抗氧化酶的活性有关.
目的 探討薑黃素(Cur)對魚籐酮(Ro)緻PC12細胞損傷的保護作用及其機製.方法 用魚籐酮建立PC12細胞損傷模型;用四氮唑鹽(MTT)比色法檢測細胞增殖活力;囌木精-伊紅染色觀察細胞形態學變化;比色法檢測細胞內總超氧化物歧化酶(SOD)的活性;DCFH-DA染色檢測細胞內活性氧類物質(ROS)水平;流式細胞術(Annexin Ⅴ-FITC/PI雙染法)檢測PC12細胞的凋亡.結果 0.5 μmol/L薑黃素和1.0 μmol/L薑黃素均可減輕0.1 μmol/L魚籐酮對PC12 細胞增殖活力的抑製,與魚籐酮組比較差異有統計學意義(P＜0.01);明顯減輕瞭細胞損傷的形態學改變;明顯增加瞭 PC12 細胞內SOD的活性,與魚籐酮組比較差異有統計學意義(P＜0.05);明顯降低瞭PC12細胞內ROS的含量,明顯抑製瞭魚籐酮對PC12 細胞凋亡的誘導作用,與魚籐酮組比較差異均有統計學意義(均P＜0.01).結論 薑黃素可拮抗魚籐酮緻PC12細胞的損傷,其機製可能與清除細胞內ROS,誘導抗氧化酶的活性有關.
목적 탐토강황소(Cur)대어등동(Ro)치PC12세포손상적보호작용급기궤제.방법 용어등동건립PC12세포손상모형;용사담서염(MTT)비색법검측세포증식활력;소목정-이홍염색관찰세포형태학변화;비색법검측세포내총초양화물기화매(SOD)적활성;DCFH-DA염색검측세포내활성양류물질(ROS)수평;류식세포술(Annexin Ⅴ-FITC/PI쌍염법)검측PC12세포적조망.결과 0.5 μmol/L강황소화1.0 μmol/L강황소균가감경0.1 μmol/L어등동대PC12 세포증식활력적억제,여어등동조비교차이유통계학의의(P＜0.01);명현감경료세포손상적형태학개변;명현증가료 PC12 세포내SOD적활성,여어등동조비교차이유통계학의의(P＜0.05);명현강저료PC12세포내ROS적함량,명현억제료어등동대PC12 세포조망적유도작용,여어등동조비교차이균유통계학의의(균P＜0.01).결론 강황소가길항어등동치PC12세포적손상,기궤제가능여청제세포내ROS,유도항양화매적활성유관.
Objective To investigate the cytoprotection of curcumin against rotenone(Ro)-induced injury and the molecular mechanisms underlying in PC12 cells.Methods The insulted model of PC12 cells was established with Ro.Cell viability was determined using MTT reduction assay.The content of reactive oxygen species(ROS)was determined by the method of DCFH-DA staining.Chromatometry was used to measure the total activity of SOD,DCFH-DA staining to measure the level of intracellular ROS,and flow cytometry to assay the apoptosis rate.Results 0.5 and 1.0 μmol/L curcumin significantly decreased the inhibitory rate of Ro on the growth of PC12 cells for 24 h as compared with the Ro group(P＜0.01).0.5 and 1.0 μmol/L curcumin significantly ameliorated the changes in morphology of PC12 cells,increased the activities of intracellular SOD as compared with the Ro group(P＜0.05),decreased the production of intracellular ROS and inhibited the apoptosis of PC12 cells induced by Ro for 24 h as compared with Ro group(P＜0.01).Conclusion Curcumin can resist Ro-induced cytotoxicity probably by the mechanism of scavenging intracellular ROS and increasing the activity of antioxidase.
