中国实验方剂学杂志
中國實驗方劑學雜誌
중국실험방제학잡지
CHINESE JOURNAL OF EXPERIMENTAL TRADITIONAL MEDICAL FORMULAE
2012年
13期
119-122
,共4页
陈云龙%卢小凤%廖锦彬%林荣锋%黄晓其%易宇阳%苏子仁
陳雲龍%盧小鳳%廖錦彬%林榮鋒%黃曉其%易宇暘%囌子仁
진운룡%로소봉%료금빈%림영봉%황효기%역우양%소자인
双黄祛毒片%没食子酸%盐酸小檗碱%大黄酸%大黄酚%芦荟大黄素%大黄素%大黄素甲醚%高效液相色谱法
雙黃祛毒片%沒食子痠%鹽痠小檗堿%大黃痠%大黃酚%蘆薈大黃素%大黃素%大黃素甲醚%高效液相色譜法
쌍황거독편%몰식자산%염산소벽감%대황산%대황분%호회대황소%대황소%대황소갑미%고효액상색보법
目的:建立测定双黄祛毒片(盐酸小檗碱、大黄、五倍子)含量的方法.方法:采用HPLC在同一色谱条件ZORBAX SB-Pheny色谱柱(4.6mm×150 mm,5μm)对没食子酸、盐酸小檗碱、大黄酸、大黄酚、芦荟大黄素、大黄素、大黄素甲醚进行含量测定.结果:线性范围分别为0.12 ～2.4 μg(r=0.999 9),盐酸小檗碱线性范围0.11 ～2.2 μg(r =0.999 9),0.015～0.3 μg(r=0.9998),0.02～0.4 μg(r=0.9998),0.008～0.16 μg(r=0.9999),0.013 ～0.26 μg(r=0.9998),0.006 ～0.12 μg(r =0.999 9),平均回收率分别为100.97％,100.20％,99.01％,99.76％,101.10％,101.97％,101.56％.结论:该方法简便、快速、准确,重复性好,可作为双黄祛毒片中没食子酸、盐酸小檗碱、大黄酸、大黄酚、芦荟大黄素、大黄素、大黄素甲醚含量测定方法.
目的:建立測定雙黃祛毒片(鹽痠小檗堿、大黃、五倍子)含量的方法.方法:採用HPLC在同一色譜條件ZORBAX SB-Pheny色譜柱(4.6mm×150 mm,5μm)對沒食子痠、鹽痠小檗堿、大黃痠、大黃酚、蘆薈大黃素、大黃素、大黃素甲醚進行含量測定.結果:線性範圍分彆為0.12 ～2.4 μg(r=0.999 9),鹽痠小檗堿線性範圍0.11 ～2.2 μg(r =0.999 9),0.015～0.3 μg(r=0.9998),0.02～0.4 μg(r=0.9998),0.008～0.16 μg(r=0.9999),0.013 ～0.26 μg(r=0.9998),0.006 ～0.12 μg(r =0.999 9),平均迴收率分彆為100.97％,100.20％,99.01％,99.76％,101.10％,101.97％,101.56％.結論:該方法簡便、快速、準確,重複性好,可作為雙黃祛毒片中沒食子痠、鹽痠小檗堿、大黃痠、大黃酚、蘆薈大黃素、大黃素、大黃素甲醚含量測定方法.
목적:건립측정쌍황거독편(염산소벽감、대황、오배자)함량적방법.방법:채용HPLC재동일색보조건ZORBAX SB-Pheny색보주(4.6mm×150 mm,5μm)대몰식자산、염산소벽감、대황산、대황분、호회대황소、대황소、대황소갑미진행함량측정.결과:선성범위분별위0.12 ～2.4 μg(r=0.999 9),염산소벽감선성범위0.11 ～2.2 μg(r =0.999 9),0.015～0.3 μg(r=0.9998),0.02～0.4 μg(r=0.9998),0.008～0.16 μg(r=0.9999),0.013 ～0.26 μg(r=0.9998),0.006 ～0.12 μg(r =0.999 9),평균회수솔분별위100.97％,100.20％,99.01％,99.76％,101.10％,101.97％,101.56％.결론:해방법간편、쾌속、준학,중복성호,가작위쌍황거독편중몰식자산、염산소벽감、대황산、대황분、호회대황소、대황소、대황소갑미함량측정방법.
