中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2008年
24期
4-6
,共3页
哮喘%细胞因子类%免疫球蛋白类
哮喘%細胞因子類%免疫毬蛋白類
효천%세포인자류%면역구단백류
Asthma%Cytokines%Immunolglobulins
目的 探讨哮喘患儿血清T细胞亚群、细胞因子和免疫球蛋白的动态变化及临床意义,为哮喘的发病机制及抗变态反应治疗提供理论依据.方法 对40例哮喘患儿(哮喘发作组和哮喘缓解组)及25例健康体检儿童(对照组)应用流式细胞仪测定CD3+、CD4+、CD8+T细胞,应用ELISA法测定TNF-α、白细胞介素(IL)-6、IL-8和IgE,应用免疫比浊法测定Igc、IgA和IgM.结果 哮喘发作组CD3+、CD4+T细胞及CD4+/CD8+.显著高于对照组(P＜0.01),CD4+T细胞及CD4+/CD8+明显高于哮喘缓解组(P＜0.05);哮喘缓解组CD4+T细胞及CD4+/CD8+明显高于对照组(P＜0.05).哮喘发作组IL-6、IL-8及TNF-α.均明显高于哮喘缓解组和对照组(P＜0.05或＜0.01).哮喘缓解组TNF-α与对照组比较差异有统计学意义(P＜0.05).哮喘发作组I-E及IgG水平显著高于哮喘缓解组和对照组(P＜0.01或＜0.05),IgA水平显著低于对照组(P＜0.01);哮喘缓解组IgE水平仍显著高于对照组(P＜0.01).结论 哮喘患儿发作期和缓解期均存在免疫失衡,提示哮喘患儿应长期抗变态反应治疗.
目的 探討哮喘患兒血清T細胞亞群、細胞因子和免疫毬蛋白的動態變化及臨床意義,為哮喘的髮病機製及抗變態反應治療提供理論依據.方法 對40例哮喘患兒(哮喘髮作組和哮喘緩解組)及25例健康體檢兒童(對照組)應用流式細胞儀測定CD3+、CD4+、CD8+T細胞,應用ELISA法測定TNF-α、白細胞介素(IL)-6、IL-8和IgE,應用免疫比濁法測定Igc、IgA和IgM.結果 哮喘髮作組CD3+、CD4+T細胞及CD4+/CD8+.顯著高于對照組(P＜0.01),CD4+T細胞及CD4+/CD8+明顯高于哮喘緩解組(P＜0.05);哮喘緩解組CD4+T細胞及CD4+/CD8+明顯高于對照組(P＜0.05).哮喘髮作組IL-6、IL-8及TNF-α.均明顯高于哮喘緩解組和對照組(P＜0.05或＜0.01).哮喘緩解組TNF-α與對照組比較差異有統計學意義(P＜0.05).哮喘髮作組I-E及IgG水平顯著高于哮喘緩解組和對照組(P＜0.01或＜0.05),IgA水平顯著低于對照組(P＜0.01);哮喘緩解組IgE水平仍顯著高于對照組(P＜0.01).結論 哮喘患兒髮作期和緩解期均存在免疫失衡,提示哮喘患兒應長期抗變態反應治療.
목적 탐토효천환인혈청T세포아군、세포인자화면역구단백적동태변화급림상의의,위효천적발병궤제급항변태반응치료제공이론의거.방법 대40례효천환인(효천발작조화효천완해조)급25례건강체검인동(대조조)응용류식세포의측정CD3+、CD4+、CD8+T세포,응용ELISA법측정TNF-α、백세포개소(IL)-6、IL-8화IgE,응용면역비탁법측정Igc、IgA화IgM.결과 효천발작조CD3+、CD4+T세포급CD4+/CD8+.현저고우대조조(P＜0.01),CD4+T세포급CD4+/CD8+명현고우효천완해조(P＜0.05);효천완해조CD4+T세포급CD4+/CD8+명현고우대조조(P＜0.05).효천발작조IL-6、IL-8급TNF-α.균명현고우효천완해조화대조조(P＜0.05혹＜0.01).효천완해조TNF-α여대조조비교차이유통계학의의(P＜0.05).효천발작조I-E급IgG수평현저고우효천완해조화대조조(P＜0.01혹＜0.05),IgA수평현저저우대조조(P＜0.01);효천완해조IgE수평잉현저고우대조조(P＜0.01).결론 효천환인발작기화완해기균존재면역실형,제시효천환인응장기항변태반응치료.
Objective To study the changes of serum levels of T-cell subgroups,cytokines and immunoglobulins on the pathogenesis of asthma in children,and explore the role of them in the pathogenesis of asthma. Methods Forty children with asthma (asthma attack group and asthma convalescence group) and 25 healthy children (control group) were enrolled in this study.The level of CD3+CD4+ and CD8+T-cell were determined using flow cytometry (FCM).The levels of TNF-α,IL-6,IL-8 and IgE were determined using ELISA, the level of IgG,IgA and IgM were detected by immunoturbidimetry.Results Compated with control group,there were higher levels of CD3+ and CD4+T-cell and CD4+/CD8+ in asthma attack group (P＜0.01),and higher levels of CD4+T-cell and CD4+/CD8+ in asthma convalescence group (P＜0.05),while no difference in the level of CD8+T-cell The level of CD4+T-cell and CD4+/CD8+ were higher in asthma convalescence group than those in control group (P＜0.05).The level so IL-6,IL-8 and TNF-αin asthma attack group were higher than those in asthma convalescence group and control group (P＜0.05 or＜0.01),the level of TNF-α was significantly higher in asthma convalescence than that in control group (P＜0.05).There were higher levels of IgE and IgG in astham attack group than those in asthma convalescence group and control group (P＜0.01 or＜0.05),the level of IgA was lower than than in conrtol group (P＜0.01).There was higher level of IgE in asthma convalescence group than that in control group (P＜0.01).Conclusions The immune imbalance exists in children with asthma at both attack and convalescence stages.A long-time anti-allergy trealment for childhood nsthma is necessary.
