CAJ | 학술논문

目的检测复发性生殖器疱疹(RGH)患者血清中可溶性PD-1(sPD-1)蛋白的浓度,探讨sPD-1的异常表达在HSV-Ⅱ病毒感染中的意义.方法选择74例RGH患者,其中发作期RGH患者34例,稳定期RGH患者40例,同时选择88例健康献血者作为对照组;应用单克隆抗体标记、ELISA法检测sPD-1的表达情况.结果 RGH组血清sPD-1蛋白水平为(33.06±17.5)μg/L,低于对照组sPD-1水平(53.07±26.3)μg/L,两者比较差异有统计学意义(t=5.78,P<0.01).发作期RGH组血清sPD-1蛋白水平为(27.47±12.9)μg/L,稳定期RGH组为(37.71±19.6)μg/L,两组间差异有统计学意义(P<0.01).结论 RGH组血清中sPD-1异常低表达,可能是HSV-Ⅱ逃避机体免疫监视、产生免疫耐受的机制之一.
목적검측복발성생식기포진(RGH)환자혈청중가용성PD-1(sPD-1)단백적농도,탐토sPD-1적이상표체재HSV-Ⅱ병독감염중적의의.방법선택74례RGH환자,기중발작기RGH환자34례,은정기RGH환자40례,동시선택88례건강헌혈자작위대조조;응용단극륭항체표기、ELISA법검측sPD-1적표체정황.결과 RGH조혈청sPD-1단백수평위(33.06±17.5)μg/L,저우대조조sPD-1수평(53.07±26.3)μg/L,량자비교차이유통계학의의(t=5.78,P<0.01).발작기RGH조혈청sPD-1단백수평위(27.47±12.9)μg/L,은정기RGH조위(37.71±19.6)μg/L,량조간차이유통계학의의(P<0.01).결론 RGH조혈청중sPD-1이상저표체,가능시HSV-Ⅱ도피궤체면역감시、산생면역내수적궤제지일.
Objective To determine the concentration of soluble PD-1 (sPD-1) in the sera of patients with recurrent genital herpes (RCH), and to explore the significance of abnormal expression of sPD-1 in RCH. Methods Serum samples were obtained from 88 healthy blood donors, 74 patients with RCH including 34 cases of outbreak-stage RCH and 40 cases of stable-stage RCH. The serum level of sPD-1 was measured by monoclonal antibody labeling and enzyme linked immunosorbent assay (ELISA). Results A significant difference was observed in the serum level of sPD-1 between the patients with RGH and the blood donors (33.06 ± 17.5 μg/L vs. 53.07 ± 26.3μg/L, P < 0.01) and between the patients with outbreak-stage RGH and those with stable-stage RGH (27.47 ± 12.9 μg/L vs. 37.71 ± 19.6 μg/L, P< 0.01). Conclusions There is a low expression of sPD-1 in patients with RGH, which may be one of the mechanisms underlying the escape of HSV- Ⅱ from immunologic surveillance and development of immunological tolerance.