CAJ | 학술논문

目的观察超宽带(ultra-wideband,UWB)电磁辐射对大鼠垂体和睾丸超微结构以及血清中性激素水平的影响。方法将SD雄性大鼠随机分为辐照组和对照组。辐照组大鼠接受脉冲数为30万次的UWB辐照，并于辐照后6、12、24、48 h,应用透射电子显微镜观察垂体及睾丸超微结构的改变，放射免疫法检测大鼠血清中睾酮(T)、黄体生成素(LH)及促卵泡激素(FSH)水平。结果在辐照后各时间点，大鼠垂体和睾丸均出现不同程度的损伤。在辐照后24h，垂体和睾丸损伤程度最重。垂体嗜碱细胞内质网扩张，胞浆中有空泡，出现脂滴，淋巴细胞渗出，染色质边集；睾丸组织中，精原细胞、支持细胞和间质细胞均出现染色质边集、内质网扩张，毛细血管内皮细胞线粒体肿胀、空泡化，淋巴细胞渗出。辐照组血清中T水平在辐照后24、48 h分别为(1209.7±115.7)、(1340.5±331.1)μg/L，明显低于相应对照组f分别为(2721.8±178.9)、(2820.9±321.4) μg/L]，差异均有统计学意义（P＜0.05），而LH和FSH均未发生明显改变。结论 UWB电磁辐射可导致大鼠垂体和睾丸超微结构发生变化，对大鼠血清中T含量有一定程度的影响，可能是UWB辐照引起大鼠生殖系统发生改变的原因之一。
목적 관찰초관대(ultra-wideband,UWB)전자복사대대서수체화고환초미결구이급혈청중성격소수평적영향。방법 장SD웅성대서수궤분위복조조화대조조。복조조대서접수맥충수위30만차적UWB복조，병우복조후6、12、24、48 h,응용투사전자현미경관찰수체급고환초미결구적개변，방사면역법검측대서혈청중고동(T)、황체생성소(LH)급촉란포격소(FSH)수평。결과 재복조후각시간점，대서수체화고환균출현불동정도적손상。재복조후24h，수체화고환손상정도최중。수체기감세포내질망확장，포장중유공포，출현지적，림파세포삼출，염색질변집；고환조직중，정원세포、지지세포화간질세포균출현염색질변집、내질망확장，모세혈관내피세포선립체종창、공포화，림파세포삼출。복조조혈청중T수평재복조후24、48 h분별위(1209.7±115.7)、(1340.5±331.1)μg/L，명현저우상응대조조f분별위(2721.8±178.9)、(2820.9±321.4) μg/L]，차이균유통계학의의（P＜0.05），이LH화FSH균미발생명현개변。결론 UWB전자복사가도치대서수체화고환초미결구발생변화，대대서혈청중T함량유일정정도적영향，가능시UWB복조인기대서생식계통발생개변적원인지일。
Objective To observe the effects of ultra-wideband (UWB) electromagnetic irradiation on the ultrastructure of pituitary or testis and the serum sex hormones level of in rats. Methods SD male rats were divided randomly into control group and irradiation groups exposed to 3×105 pulses UWB irradiation for 6,12, 24, 48 h. After exposure, the ultrastructure changes of pituitary and testis were observed by electron microscope. Serum testosterone (T) , luteinizing hormone (LH) and follicle stimulating hormone (FSH) were measured by radioimmunoassay. Results The rat pituitary and testis were injured at the different time after exposure to UWB electromagnetic irradiation, but the damage was serious at the 24 h after exposure. In the basophilic cell of the pituitary, there were the vacuoles and lipid droplets, dilated endoplasmic reticulum (ER),exuded lymphocytes and gathered chromatin in the cell border. In the testis tissue, there were the dilated ER and gathered chromatin in the cell border of the spermatogonium, interstitial cell and sustentacular cell, some mitochondria became vacuolar and swollen in the capillary endotheliocytes and exuded lymphocytes. Serum Tlevels of the irradiation groups at 24 and 48 h were ( 1209.7±115.7 ), ( 1340.5±331.1 ) μg/L, which were significantly lower than those[(2721.8± 178.9) and ( 2820.9±321.4 ) μg/L]of control groups at 24 and 48 h (P＜0.05). There was no significant difference for LH and FSH between exposure groups and control groups. Conclusions UWB electromagnetic radiation could induce some changes in the ultrastructure of rat pituitary and testis and serum T level, which may induce the change of reproductive system.