CAJ | 학술논문

目的探讨胃癌组织中膜型基质金属蛋白酶-1 (MT1-MMP)和RECK蛋白表达状况和两者之间的关系,及其表达与胃癌临床诊断之间的关系.方法采用免疫组织化学法(两步法)对胃癌切除标本进行研究.结果在44例胃癌标本中,有37(84.1％)例MT1-MMP表达呈阳性反应,31(70.5％)例RECK免疫组化为阳性.MT1-MMP表达与胃癌分化具有一定的关系,低分化组织标本中,MT1 -MMP表达较多,而中、高分化组织标本中,MT1 -MMP表达相对较弱,具有统计学意义(P=0.015).胃癌中MT1-MMP表达与肿瘤细胞浸润深度相关(P=0.007).但MT1-MMP表达与性别以及有无淋巴结转移之间未见统计学差异.与此相反,在胃癌标本中,高分化标本中RECK蛋白表达相对较多,中分化标本其次,在低分化标本中表达较少.RECK表达与肿瘤分化具有统计学意义(P=0.006).RECK表达与性别、肿瘤细胞浸润深度以及有无淋巴结转移无关,且与MT1-MMP表达之间亦无统计学意义.结论 MT1-MMP过表达在胃癌分化、转移中发挥着重要的作用,而RECK的表达有益于胃癌向高分化发展.
목적 탐토위암조직중막형기질금속단백매-1 (MT1-MMP)화RECK단백표체상황화량자지간적관계,급기표체여위암림상진단지간적관계.방법 채용면역조직화학법(량보법)대위암절제표본진행연구.결과 재44례위암표본중,유37(84.1％)례MT1-MMP표체정양성반응,31(70.5％)례RECK면역조화위양성.MT1-MMP표체여위암분화구유일정적관계,저분화조직표본중,MT1 -MMP표체교다,이중、고분화조직표본중,MT1 -MMP표체상대교약,구유통계학의의(P=0.015).위암중MT1-MMP표체여종류세포침윤심도상관(P=0.007).단MT1-MMP표체여성별이급유무림파결전이지간미견통계학차이.여차상반,재위암표본중,고분화표본중RECK단백표체상대교다,중분화표본기차,재저분화표본중표체교소.RECK표체여종류분화구유통계학의의(P=0.006).RECK표체여성별、종류세포침윤심도이급유무림파결전이무관,차여MT1-MMP표체지간역무통계학의의.결론 MT1-MMP과표체재위암분화、전이중발휘착중요적작용,이RECK적표체유익우위암향고분화발전.
Objective To examine the expression of membrane-type-1 matrix metalloproteinase (MT1-MMP) and reversion-inducing cysteine-rich protein with Kazal motifs (RECK) in gastric carcinoma,and investigate its clinical significance,at the same time analyze the correlation between MT1-MMP and RECK expression.Methods MT1-MMP and RECK expression in surgically resected tissue samples of gastric carcinoma was examined by immunohistochemical method (two-step method),and its correlation with clinicopathological factors was analyzed.Results Among the 44 gastric carcinoma samples,37 (84.1％ )were stained positive for MT1-MMP,and 31(70.5％ ) for RECK.The expression of MT1-MMP was much higher in poorly differentiated gastric carcinoma samples than moderately and well-differentiated samples (P =0.015).The expression level of MT1-MMP was associated with invasive depth of tumor cells (P =0.007),but no difference between sex and lymph node metastasis.On the contrary,the well-differentiated samples showed higher expression of RECK than poorly and moderately differentiated gastric carcinoma samples (P =0.006).The expression level of RECK did not correlate with sex,lymph node metastasis and invasive depth of tumor cells.RECK expression showed no relation to MT1-MMP expression in the gastric carcinoma.Conclusion Overexpression of MT1-MMP in gastric carcinoma may play an important role during tumor differentiation and metastasis,the RECK protein may have positive effects on the tumor differentiation.