CAJ | 학술논문

目的探讨转化生长因子-β1 (TGF-β1)诱导胃癌细胞的凋亡作用及机制.方法利用TGF-β1诱导胃癌细胞SGC7901凋亡,小干扰RNA(siRNA)封闭半胱氨酰天冬氨酸特异性蛋白酶(Caspase)-8的表达,Western blot检测诱导及封闭前后各Caspases表达随时间的变化,流式细胞术分析细胞的凋亡率.结果 TGF-β1作用后,各组细胞的凋亡率明显增加,0、24、48 h凋亡率分别为1.13％、14.88％、37.32％；Caspase-8 12 h后激活,Caspase-9 24 h后激活；白RNA干扰(RNAi)后Caspase-8表达明显下降,24 h后Caspase-8表达量为对照组的11.2％,48～ 72 h未测到表达,96 h后Caspase-8表达重新出现,为对照组的9.2％.结论 TGF-β1通过Caspase途径诱导胃癌细胞发生凋亡,由Caspase-8开始启动；抑制Caspase-8表达可以在一定程度上阻断TGF-β1/Caspases信号的转导,进而抑制细胞凋亡.
목적 탐토전화생장인자-β1 (TGF-β1)유도위암세포적조망작용급궤제.방법 이용TGF-β1유도위암세포SGC7901조망,소간우RNA(siRNA)봉폐반광안선천동안산특이성단백매(Caspase)-8적표체,Western blot검측유도급봉폐전후각Caspases표체수시간적변화,류식세포술분석세포적조망솔.결과 TGF-β1작용후,각조세포적조망솔명현증가,0、24、48 h조망솔분별위1.13％、14.88％、37.32％；Caspase-8 12 h후격활,Caspase-9 24 h후격활；백RNA간우(RNAi)후Caspase-8표체명현하강,24 h후Caspase-8표체량위대조조적11.2％,48～ 72 h미측도표체,96 h후Caspase-8표체중신출현,위대조조적9.2％.결론 TGF-β1통과Caspase도경유도위암세포발생조망,유Caspase-8개시계동；억제Caspase-8표체가이재일정정도상조단TGF-β1/Caspases신호적전도,진이억제세포조망.
Objective To investigate the effects of transforming growth factor-β1 (TGF-β1) induced apoptosis.Methods Apoptosis proportion of human gastric SGC7901 carcinoma cells induced by TGF-β1 was tested by flow cytometry,blocking Caspase-8 by RNA interference (RNAi) ,the expression of Caspase-8 was analyzed by Western blotting.Results The apoptosis rate of SGC7901 cells obviously increased with the treatment of TGF-β1,an active fragment of caspase-8 started to occur 12 h after TGF-β1 treatment,whereas the caspase-9 active fragment occurred 24 h after TGF-β1 treatment. After RNAi against Caspase-8,the expression of Caspase-8 was decreased,11.2％ of control group at 24 h,and then can not be detected at 48 h,72 h,re-emergence at 96 h,9.2％ of control group.Conclusion TGF-β1 induced the apoptosis of SGC7901 cells via Caspases pathway,caspase-8 may be the first caspase activated;Suppression of caspase-8 by RNAi can effectively block the TGF-β1/Caspases signal pathway,and subsequently inhibits the apoptosis of SGC7901 cells.