中华心血管病杂志
中華心血管病雜誌
중화심혈관병잡지
Chinese Journal of Cardiology
2011年
4期
301-304
,共4页
周青%王天杰%张存泰%阮磊%李连东%徐仁德%全小庆%倪明科
週青%王天傑%張存泰%阮磊%李連東%徐仁德%全小慶%倪明科
주청%왕천걸%장존태%원뢰%리련동%서인덕%전소경%예명과
心动过速,室性%连接蛋白类%抗心律失常药%溶血磷脂酸
心動過速,室性%連接蛋白類%抗心律失常藥%溶血燐脂痠
심동과속,실성%련접단백류%항심률실상약%용혈린지산
Tachycardia,ventricular%Connexins%Anti-arrhythmia agents%Lysophosphatidic acid
目的 探讨抗心律失常肽(antiarrhythmic petide 10,AAP10)和溶血磷脂酸(lysophosphatidic acid,LPA)对兔室性心律失常的影响及其作用机制.方法 24只新西兰大白兔随机分为正常对照组、LPA组和AAP10干预组,建立兔左心室楔形心肌块模型,同步记录楔形心肌块内、外膜心肌细胞动作电位和跨室壁心电图,程序电刺激起搏,记录室性心律失常发生率.通过免疫印迹技术(Western blot)检测三组心肌去磷酸化缝隙连接蛋白43(non-phosphorylated connexin 43,NPCx43)的变化,采用免疫荧光技术观察NP-Cx43的分布.结果 LPA组QT间期、动作电位复极90%时程(90%of duration of action potential,APD90)、跨室壁复极离散度(transmural repolarization dispersion,TDR)与对照组相比均明显增加(P<0.01),同时多形性室性心动过速的诱发率也明显增加(62.5%比0,P<0.01),并伴随NP-Cx43含量明显增加(P<0.01);AAP10干预组与LPA组相比,QT间期、内膜APD90、TDR均明显缩小(P<0.01),多形性室性心动过速的诱发率也明显降低(P<0.05),NP-Cx43含量也明显下降(P<0.01).三组之间总Cx43含量差异无统计学意义.结论LPA有明显致心律失常作用,其机制可能与引起NP-Gx43含量增加、抑制缝隙连接通道功能有关,而AAP10可以拮抗LPA引起的NP-Cx43含量增加,同时降低LPA所致室性心律失常的发生率,具有抗心律失常的保护作用.
目的 探討抗心律失常肽(antiarrhythmic petide 10,AAP10)和溶血燐脂痠(lysophosphatidic acid,LPA)對兔室性心律失常的影響及其作用機製.方法 24隻新西蘭大白兔隨機分為正常對照組、LPA組和AAP10榦預組,建立兔左心室楔形心肌塊模型,同步記錄楔形心肌塊內、外膜心肌細胞動作電位和跨室壁心電圖,程序電刺激起搏,記錄室性心律失常髮生率.通過免疫印跡技術(Western blot)檢測三組心肌去燐痠化縫隙連接蛋白43(non-phosphorylated connexin 43,NPCx43)的變化,採用免疫熒光技術觀察NP-Cx43的分佈.結果 LPA組QT間期、動作電位複極90%時程(90%of duration of action potential,APD90)、跨室壁複極離散度(transmural repolarization dispersion,TDR)與對照組相比均明顯增加(P<0.01),同時多形性室性心動過速的誘髮率也明顯增加(62.5%比0,P<0.01),併伴隨NP-Cx43含量明顯增加(P<0.01);AAP10榦預組與LPA組相比,QT間期、內膜APD90、TDR均明顯縮小(P<0.01),多形性室性心動過速的誘髮率也明顯降低(P<0.05),NP-Cx43含量也明顯下降(P<0.01).三組之間總Cx43含量差異無統計學意義.結論LPA有明顯緻心律失常作用,其機製可能與引起NP-Gx43含量增加、抑製縫隙連接通道功能有關,而AAP10可以拮抗LPA引起的NP-Cx43含量增加,同時降低LPA所緻室性心律失常的髮生率,具有抗心律失常的保護作用.
목적 탐토항심률실상태(antiarrhythmic petide 10,AAP10)화용혈린지산(lysophosphatidic acid,LPA)대토실성심률실상적영향급기작용궤제.방법 24지신서란대백토수궤분위정상대조조、LPA조화AAP10간예조,건립토좌심실설형심기괴모형,동보기록설형심기괴내、외막심기세포동작전위화과실벽심전도,정서전자격기박,기록실성심률실상발생솔.통과면역인적기술(Western blot)검측삼조심기거린산화봉극련접단백43(non-phosphorylated connexin 43,NPCx43)적변화,채용면역형광기술관찰NP-Cx43적분포.결과 LPA조QT간기、동작전위복겁90%시정(90%of duration of action potential,APD90)、과실벽복겁리산도(transmural repolarization dispersion,TDR)여대조조상비균명현증가(P<0.01),동시다형성실성심동과속적유발솔야명현증가(62.5%비0,P<0.01),병반수NP-Cx43함량명현증가(P<0.01);AAP10간예조여LPA조상비,QT간기、내막APD90、TDR균명현축소(P<0.01),다형성실성심동과속적유발솔야명현강저(P<0.05),NP-Cx43함량야명현하강(P<0.01).삼조지간총Cx43함량차이무통계학의의.결론LPA유명현치심률실상작용,기궤제가능여인기NP-Gx43함량증가、억제봉극련접통도공능유관,이AAP10가이길항LPA인기적NP-Cx43함량증가,동시강저LPA소치실성심률실상적발생솔,구유항심률실상적보호작용.
Objective To investigate the effect and potential mechanism of lysophosphatidic acid (LPA) and antiarrhythmic peptide (AAP10) on rabbit ventricular arrhythmia. Methods Twenty-four rabbits were randomly divided into three groups (n =8 each): control group, LPA group and AAP10 + LPA group. Using arterially perfused rabbit ventricular wedge preparations, transmural ECG and action potentials from both endocardium and epicardium were simultaneously recorded in the whole process of all experiments with two separate floating microeletrodes. The incidence of ventricular arrhythmia post S1S2 stimulation was recorded. Protein levels of nonphosphorylated Cx43 and total Cx43 were evaluated by Western blot. The distribution of nonphosphorylated Cx43 was observed by confocal immunofluorescence microscopy. Results Compared with the control group, the QT interval, endocardial action potential duration, transmural repolarization dispersion (TDR) and incidence of ventricular arrhythmia were significantly increased and nonphosphorylated Cx43 expression was significantly upregulated in the LPA group. Compared with the LPA group, cotreatment with AAP10 can reduce the QT interval, endocardial action potential duration, TDR and incidence of ventricular arrhythmia (25.0% vs 62. 5%, P < 0. 01 ) and downregulate nonphosphorylated Cx43. Conclusions LPA could promote the arrhythmia possibly by upregulating nonphosphorylated Cx43 and subsequent gap junction transmission inhibition. Gap junction enhancer AAP10 could attenuate the proarrhythmic effect of LPA probably by downregulating myocardial nonphosphorylated Cx43 expression.