中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2009年
5期
499-503
,共5页
夏昌宇%刘岩%郭红雁%孙桂珍%黄宪章%庄俊华%于琴%刘牧龙%王兰珍%张雪莲%徐国宾
夏昌宇%劉巖%郭紅雁%孫桂珍%黃憲章%莊俊華%于琴%劉牧龍%王蘭珍%張雪蓮%徐國賓
하창우%류암%곽홍안%손계진%황헌장%장준화%우금%류목룡%왕란진%장설련%서국빈
丙氨酸转氨酶%天冬氨酸氨基转移酶类%磷酸毗哆醛%临床酶试验%参考标准%评价研究
丙氨痠轉氨酶%天鼕氨痠氨基轉移酶類%燐痠毗哆醛%臨床酶試驗%參攷標準%評價研究
병안산전안매%천동안산안기전이매류%린산비치철%림상매시험%삼고표준%평개연구
Alanine transaminase%Apartate aminotransferase%Pyridoxal phosphate%Clinical enzyme tests%Reference standards%Evaluation studies
目的 调查我国4家参考实验室应用国际临床化学联合会(IFCC)推荐加和不加磷酸吡哆醛参考方法测定ALT和AST的室内和室间变异,为设定合适的室内和室间变异的范围提供依据.方法 建立加和不加磷酸毗哆醛的IFCC方法,用均一性良好的5个浓度冰冻混合人血清作为比对材料,观察各实验室测量比对样本的室内和室间变异,并与2006年和2007年参考实验室外部质量评价计划(RALE)的结果做比较.同时分析加与不加磷酸吡哆醛时AST、ALT测定值及AST/ALT比值的差异.结果 本次比对的室间变异大于室内变异,仅个别实验室在个别结果出现室内变异大于室间变异的情况.室内变异不大于RELA比对室内变异的变化,室间变异亦明显小于后者.加磷酸吡哆醛法的ALT和AST测量值均比未加法高,两种方法得到的AST/ALT比值具有显著性差异.结论 建议酶学参考实验室ALT、AST测定室内变异分别小于2.72%、2.58%;使用冰冻血清作比对材料时,二者的室间变异小于3.5%;冰冻干燥品作比对材料时,二者的室间变异小于4.5%,应观察不同个体样本加和不加磷酸吡哆醛转氨酶测定值及其比值的变化在疾病诊断与预后评估中的临床意义.
目的 調查我國4傢參攷實驗室應用國際臨床化學聯閤會(IFCC)推薦加和不加燐痠吡哆醛參攷方法測定ALT和AST的室內和室間變異,為設定閤適的室內和室間變異的範圍提供依據.方法 建立加和不加燐痠毗哆醛的IFCC方法,用均一性良好的5箇濃度冰凍混閤人血清作為比對材料,觀察各實驗室測量比對樣本的室內和室間變異,併與2006年和2007年參攷實驗室外部質量評價計劃(RALE)的結果做比較.同時分析加與不加燐痠吡哆醛時AST、ALT測定值及AST/ALT比值的差異.結果 本次比對的室間變異大于室內變異,僅箇彆實驗室在箇彆結果齣現室內變異大于室間變異的情況.室內變異不大于RELA比對室內變異的變化,室間變異亦明顯小于後者.加燐痠吡哆醛法的ALT和AST測量值均比未加法高,兩種方法得到的AST/ALT比值具有顯著性差異.結論 建議酶學參攷實驗室ALT、AST測定室內變異分彆小于2.72%、2.58%;使用冰凍血清作比對材料時,二者的室間變異小于3.5%;冰凍榦燥品作比對材料時,二者的室間變異小于4.5%,應觀察不同箇體樣本加和不加燐痠吡哆醛轉氨酶測定值及其比值的變化在疾病診斷與預後評估中的臨床意義.
목적 조사아국4가삼고실험실응용국제림상화학연합회(IFCC)추천가화불가린산필치철삼고방법측정ALT화AST적실내화실간변이,위설정합괄적실내화실간변이적범위제공의거.방법 건립가화불가린산비치철적IFCC방법,용균일성량호적5개농도빙동혼합인혈청작위비대재료,관찰각실험실측량비대양본적실내화실간변이,병여2006년화2007년삼고실험실외부질량평개계화(RALE)적결과주비교.동시분석가여불가린산필치철시AST、ALT측정치급AST/ALT비치적차이.결과 본차비대적실간변이대우실내변이,부개별실험실재개별결과출현실내변이대우실간변이적정황.실내변이불대우RELA비대실내변이적변화,실간변이역명현소우후자.가린산필치철법적ALT화AST측량치균비미가법고,량충방법득도적AST/ALT비치구유현저성차이.결론 건의매학삼고실험실ALT、AST측정실내변이분별소우2.72%、2.58%;사용빙동혈청작비대재료시,이자적실간변이소우3.5%;빙동간조품작비대재료시,이자적실간변이소우4.5%,응관찰불동개체양본가화불가린산필치철전안매측정치급기비치적변화재질병진단여예후평고중적림상의의.
Objective To investigate the intralaboratury and interlabomtory variations of measurements for ALT and AST among four domestic reference laboratories. Methods The International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) reference procedures and IFCC procedures without pyridoxal 5-phosphate (PLP) were performed in the reference laboratories. Intralaboratory and interlaboratory CVs were compared with those in 2006 and 2007 IFCC External Quality Assessment Scheme for Reference Laboratories (RELA). Meanwhile, deviations of results for ALT, AST and AST/ALT between two methods were calculated. Results Interlaboratory CVs were generally higher than intralaboratory CVs. Interlaboratory CVs among the 41 laboratories were lower than these in RELA. Results of ALT and AST using method with PLP were higher than those using method without PLP. Difference of AST/ALT ratio between the two methods was significant. Conclusions For reference measurement of the 2 enzymes, interlaboratory CVs of < 3.5 are achievable on frozen serum materials. Measurements on lyophilized materials may have higher CVs. Further studies are needed for the investigation of the differences between results obtained in the absence and presence of PLP.
