中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2011年
10期
1097-1099
,共3页
王继琛%包世新%鲍缦夕%李海
王繼琛%包世新%鮑縵夕%李海
왕계침%포세신%포만석%리해
小窝蛋白-1%骨肉瘤%免疫组织化学
小窩蛋白-1%骨肉瘤%免疫組織化學
소와단백-1%골육류%면역조직화학
Caveolin-1%Osteosarcoma%Immunohistochemistry
目的 通过检测小窝蛋白-1在人骨肉瘤组织中的表达,探讨小窝蛋白-1在骨肉瘤发生、发展及转移中的作用及其与临床病理相关性.方法 采用免疫组化学方法检测48例骨肉瘤组织标本及24例正常骨组织中小窝蛋白-1的表达水平.并结合临床、病理特征进行分析.结果 48例骨肉瘤组织中有38例小窝蛋白-1呈弱表达或未见表达,而在24例正常的骨组织标本中小窝蛋白-1均有表达且呈强表达,骨肉瘤组织、正常骨组织中小窝蛋白-1的平均吸光度值分别为(0.2292±0.0329)和(0.6428±0.0028),骨肉瘤组织小窝蛋白-1的表达明显低于正常骨组织(t=21.48,P＜0.01),差异具有统计学意义；小窝蛋白-1的表达与患者年龄、性别、肿瘤大小、病理分级等均无相关性(P均＞0.05),而小窝蛋白-1的低表达或不表达与肺转移有关(x2 =11.84,P＜0.05).结论 小窝蛋白-1异常低表达可能参与骨肉瘤侵袭、转移机制,小窝蛋白-1可能成为判断骨肉瘤患者预后新的分子标志物及肿瘤治疗的新靶点.
目的 通過檢測小窩蛋白-1在人骨肉瘤組織中的錶達,探討小窩蛋白-1在骨肉瘤髮生、髮展及轉移中的作用及其與臨床病理相關性.方法 採用免疫組化學方法檢測48例骨肉瘤組織標本及24例正常骨組織中小窩蛋白-1的錶達水平.併結閤臨床、病理特徵進行分析.結果 48例骨肉瘤組織中有38例小窩蛋白-1呈弱錶達或未見錶達,而在24例正常的骨組織標本中小窩蛋白-1均有錶達且呈彊錶達,骨肉瘤組織、正常骨組織中小窩蛋白-1的平均吸光度值分彆為(0.2292±0.0329)和(0.6428±0.0028),骨肉瘤組織小窩蛋白-1的錶達明顯低于正常骨組織(t=21.48,P＜0.01),差異具有統計學意義；小窩蛋白-1的錶達與患者年齡、性彆、腫瘤大小、病理分級等均無相關性(P均＞0.05),而小窩蛋白-1的低錶達或不錶達與肺轉移有關(x2 =11.84,P＜0.05).結論 小窩蛋白-1異常低錶達可能參與骨肉瘤侵襲、轉移機製,小窩蛋白-1可能成為判斷骨肉瘤患者預後新的分子標誌物及腫瘤治療的新靶點.
목적 통과검측소와단백-1재인골육류조직중적표체,탐토소와단백-1재골육류발생、발전급전이중적작용급기여림상병리상관성.방법 채용면역조화학방법검측48례골육류조직표본급24례정상골조직중소와단백-1적표체수평.병결합림상、병리특정진행분석.결과 48례골육류조직중유38례소와단백-1정약표체혹미견표체,이재24례정상적골조직표본중소와단백-1균유표체차정강표체,골육류조직、정상골조직중소와단백-1적평균흡광도치분별위(0.2292±0.0329)화(0.6428±0.0028),골육류조직소와단백-1적표체명현저우정상골조직(t=21.48,P＜0.01),차이구유통계학의의；소와단백-1적표체여환자년령、성별、종류대소、병리분급등균무상관성(P균＞0.05),이소와단백-1적저표체혹불표체여폐전이유관(x2 =11.84,P＜0.05).결론 소와단백-1이상저표체가능삼여골육류침습、전이궤제,소와단백-1가능성위판단골육류환자예후신적분자표지물급종류치료적신파점.
Objective By detecting the expression of caveolin-1 in osteosarcoma tissues,to explore its possible roles in the occurrence,progress and metastasis of osteosarcoma and its relationship with clinicopathological features.Methods The expression level of caveolin-1 protein in 48 osteosarcoma tissues and 24 normal bone tissues were detected by immunohistochemistry of SP method.Its relationship with clinical and pathological features were also analyzed.Results among the 48 osteosarcomas examined,38 were stained weak or negative for caveolin-1,whereas the strong caveolin-1 staining was observed in all the 24 normal bone tissues.The absorbent optical density value of caveolin-1 protein in osteosarcoma tissues and normal bone tissues were(0.2292 ± 0.0329) and(0.6428 ± 0.0028),respectively.The expression of caveolin-1 in osteosarcoma tissues was significantly lower than that in normal bone tissues (t =21.48,P ＜ 0.01 ).Caveolin-1 protein expression was significant correlated with the pulmonary metastasis of tumors ( t =11.84,P ＜ 0.05 ),but had no correlation with nge,sex,tumor size and pathologic grades.Conclusion The low expression of caveolin-1 may participate in osteosarcoma invasion and metastasis,and may be a new prognostic molecular marker for osteosarcoma and a new therapeutic target for tumor.
