中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2011年
4期
280-283
,共4页
周从阳%张志坚%罗雅娟%肖声平%林时荣
週從暘%張誌堅%囉雅娟%肖聲平%林時榮
주종양%장지견%라아연%초성평%림시영
百草枯%乌司他丁%热休克蛋白质类70%NF-кb
百草枯%烏司他丁%熱休剋蛋白質類70%NF-кb
백초고%오사타정%열휴극단백질류70%NF-кb
Paraquat%Ulilnastatin%Heat shock proteins70%NF-κB
目的 观察急性百草枯(paraquat,PQ)染毒大鼠肺组织中热休克蛋白70(hsp70)及NF-κB mRNA表达及乌司他丁(ulilnastatin,UTI)的作用.方法 将72只SD大鼠随机分为对照组、PQ组和UTI组,每组24只.染毒组:以80 mg/lg PQ一次性灌胃;治疗组:PQ灌胃后以UTI 10000 U/kg腹腔注射,每日1次.对照组一次性灌胃生理盐水.于12、24、48、72 h分别测定肺组织中髓过氧化物酶(MPO)活力,反转录-聚合酶链反应(RT-PCR)法测定肺组织中hsp70和NF-κB p65 mRNA的表达,观察大鼠肺组织病理变化.结果 PQ染毒后48 h内炎性细胞浸润明显,伴充血水肿;UTI组炎性细胞浸润较PQ染毒组少,充血水肿轻.染毒组大鼠肺组织中MPO活力在12、24、48、72 h分别为(31.72±6.42)、(56.23±8.63)、(87.21±10.02)、(107.21±13.52)U/g,UTI组12、24、48、72 h MPO活力分别为(15.65±3.21)、(35.98±5.74)、(59.33±9.65)、(71.25±10.58)U/g,较对照组[(11.38±1.25)U/g]明显升高,差异有统计学意义(P＜0.01);UTI组MPO活力较染毒组明显降低,差异有统计学意义(P＜0.01).UTI组12、24、48、72 h NF-κB p65 mRNA表达分别为0.3288±0.0147、0.5337±0.0328、0.7357±0.0424、0.7547±0.0905,与PQ组(分别为0.41 85±0.0294、0.8532±0.0841、0.9554±0.0975、1.0094±0.0703)比较,明显降低,差异有统计学意义(P＜0.01).UTI组12、24、48、72 h hsp70 mRNA表达分别为0.5193±0.0254、0.8289±0.0606、0.7566±0.0277、0.4873±0.0105,与PQ组(分别为0.3897±0.0125、0.5904±0.0186、0.4007±0.0237、0.2293±0.0137)比较明显增加,差异有统计学意义(P＜0.01).结论 大鼠急性PQ中毒后肺组织中hsp70、NF-κB p65 mRNA表达明显增高;UTI可以通过增加PQ中毒大鼠肺组织中HSP70表达,降低NF-κB表达,对肺脏起保护作用.
目的 觀察急性百草枯(paraquat,PQ)染毒大鼠肺組織中熱休剋蛋白70(hsp70)及NF-κB mRNA錶達及烏司他丁(ulilnastatin,UTI)的作用.方法 將72隻SD大鼠隨機分為對照組、PQ組和UTI組,每組24隻.染毒組:以80 mg/lg PQ一次性灌胃;治療組:PQ灌胃後以UTI 10000 U/kg腹腔註射,每日1次.對照組一次性灌胃生理鹽水.于12、24、48、72 h分彆測定肺組織中髓過氧化物酶(MPO)活力,反轉錄-聚閤酶鏈反應(RT-PCR)法測定肺組織中hsp70和NF-κB p65 mRNA的錶達,觀察大鼠肺組織病理變化.結果 PQ染毒後48 h內炎性細胞浸潤明顯,伴充血水腫;UTI組炎性細胞浸潤較PQ染毒組少,充血水腫輕.染毒組大鼠肺組織中MPO活力在12、24、48、72 h分彆為(31.72±6.42)、(56.23±8.63)、(87.21±10.02)、(107.21±13.52)U/g,UTI組12、24、48、72 h MPO活力分彆為(15.65±3.21)、(35.98±5.74)、(59.33±9.65)、(71.25±10.58)U/g,較對照組[(11.38±1.25)U/g]明顯升高,差異有統計學意義(P＜0.01);UTI組MPO活力較染毒組明顯降低,差異有統計學意義(P＜0.01).UTI組12、24、48、72 h NF-κB p65 mRNA錶達分彆為0.3288±0.0147、0.5337±0.0328、0.7357±0.0424、0.7547±0.0905,與PQ組(分彆為0.41 85±0.0294、0.8532±0.0841、0.9554±0.0975、1.0094±0.0703)比較,明顯降低,差異有統計學意義(P＜0.01).UTI組12、24、48、72 h hsp70 mRNA錶達分彆為0.5193±0.0254、0.8289±0.0606、0.7566±0.0277、0.4873±0.0105,與PQ組(分彆為0.3897±0.0125、0.5904±0.0186、0.4007±0.0237、0.2293±0.0137)比較明顯增加,差異有統計學意義(P＜0.01).結論 大鼠急性PQ中毒後肺組織中hsp70、NF-κB p65 mRNA錶達明顯增高;UTI可以通過增加PQ中毒大鼠肺組織中HSP70錶達,降低NF-κB錶達,對肺髒起保護作用.
목적 관찰급성백초고(paraquat,PQ)염독대서폐조직중열휴극단백70(hsp70)급NF-κB mRNA표체급오사타정(ulilnastatin,UTI)적작용.방법 장72지SD대서수궤분위대조조、PQ조화UTI조,매조24지.염독조:이80 mg/lg PQ일차성관위;치료조:PQ관위후이UTI 10000 U/kg복강주사,매일1차.대조조일차성관위생리염수.우12、24、48、72 h분별측정폐조직중수과양화물매(MPO)활력,반전록-취합매련반응(RT-PCR)법측정폐조직중hsp70화NF-κB p65 mRNA적표체,관찰대서폐조직병리변화.결과 PQ염독후48 h내염성세포침윤명현,반충혈수종;UTI조염성세포침윤교PQ염독조소,충혈수종경.염독조대서폐조직중MPO활력재12、24、48、72 h분별위(31.72±6.42)、(56.23±8.63)、(87.21±10.02)、(107.21±13.52)U/g,UTI조12、24、48、72 h MPO활력분별위(15.65±3.21)、(35.98±5.74)、(59.33±9.65)、(71.25±10.58)U/g,교대조조[(11.38±1.25)U/g]명현승고,차이유통계학의의(P＜0.01);UTI조MPO활력교염독조명현강저,차이유통계학의의(P＜0.01).UTI조12、24、48、72 h NF-κB p65 mRNA표체분별위0.3288±0.0147、0.5337±0.0328、0.7357±0.0424、0.7547±0.0905,여PQ조(분별위0.41 85±0.0294、0.8532±0.0841、0.9554±0.0975、1.0094±0.0703)비교,명현강저,차이유통계학의의(P＜0.01).UTI조12、24、48、72 h hsp70 mRNA표체분별위0.5193±0.0254、0.8289±0.0606、0.7566±0.0277、0.4873±0.0105,여PQ조(분별위0.3897±0.0125、0.5904±0.0186、0.4007±0.0237、0.2293±0.0137)비교명현증가,차이유통계학의의(P＜0.01).결론 대서급성PQ중독후폐조직중hsp70、NF-κB p65 mRNA표체명현증고;UTI가이통과증가PQ중독대서폐조직중HSP70표체,강저NF-κB표체,대폐장기보호작용.
Objective To observe the expression levels of heat shock protein70 (hsp70) and NF-κB p65 mRNA in lung tissue of acute paraquat (PQ) poisoning rats, and intervention effects of ulinastatin (UTI).Methods Seventy-two Sprague-Dawley (SD) rats were randomly divided into three groups: PQ poisoning group, UTI group and control group. The rats were exposed intragastrically to PQ at the dose of 80 mg/kg to establish a model of the rat acute lung injury. The UTI group was intervened by peritoneal injection with 10000 U/kg UTI in 30 minutes. On the 12, 24, 48, 72 h after exposure, myeloperoxidase (MPO) activity in lung tissue were detected. The expression of the NF-κBp 65 mRNA and hsp70 mRNA in lung tissue was detected by the reverse transcription-PCR (RT-PCR).The lung pathological changes of rats were observed. Results The degree of lung injury in PQ group and UTI group was higher than that in control group. But in UTI group the degree of lung injury was lower than PQ group. MPO activity in the lung tissues in PQ group was (31.72±6.42),(56.23±8.63), (87.21 ±10.02) and (107.21 ±13.52) μ/g in 12, 24, 48 and 72 h, respectively which was significantly higher than that [(11.38±1.25) μ/g] in control group (P＜0.01). MPO activity in the lung tissues in UTI group was (15.65±3.21), (35.98±5.74), (59.33±9.65) and (71.25±10.58) μ/g in 12, 24, 48 and 72 h,respectively which was significantly lower than those in PQ group (P＜0.01). The expression levels of NF-κB p65 mRNA of lung tissues in UTI group in 12, 24, 48 and 72 h were 0.3288±0.0147, 0.5337±0.0328, 0.7357±0.0424 and 0.7547±0.0905, respectively, which were significantly lower that those (0.4185±0.0294, 0.8532±0.0841,0.9554±0.0975 and 1 .0094±0.0703) in PQ group (P＜0.01). hsp70 mRNA expression levels in 12, 24,48 and 72 h of the UTI group were 0.5193±0.0254, 0.8289±0.0606, 0.7566±0.0277 and 0.4873±0.0105,respectively, which were significantly higher than those (0.3897±0.0125, 0.5904±0.0186, 0.4007±0.0237 and 0.2293±0.0137) in PQ group (P＜0.01). Conclusion The expression levels of hsp70 mRNA and NF-κB p65 mRNA of rats after intoxication increased significantly. UTI can protect the lung tissus by elevating the expression of hsp70 and reducing the expression of N F-κB in the lung tissues of rats with acute paraquat poisoning.
