CAJ | 학술논문

目的观察葛根素注射液联合厄贝沙坦对心室快速起搏致充血性心力衰竭(congestive heartfailure,CHF)实验犬心房颤动及心房纤维化的影响.方法选择健康成年杂种犬21只,随机令为正常组对照组、CI-IF模型组和TMP治疗组.采用右心室快速起搏建立实验犬CHF模型.Butst刺激诱发心房颤动(atrial fibrill;tripn,LVEF).超声心动图仪检测实验犬左心室射血分数(1eft ventricular ejection fraction,LVEF).Mallory's三色法染色检测心房组织纤维比程度.采用放射免疫法测定血桨血管紧张素1[和醛固酮的浓度,测定血清m型前肢原氨基末端肽(amino-terllllrlal1～eptldcoftypeⅢprocollagen,PⅢ NP)、层粘连蛋白(1alTlmln,l.N)和透明质酸(hyaltlronlcacid,HA)的水平.结果 CHF模型组LVEF较正常对照组明显下降(P＜0.01);AF发生率、持续性AF发生率及AF持续时间较正常对照组均明显增加(P＜0.01);左右心房纤维化程度较正常对照组亦有明显增加(P＜0.01);AF持续时间与左心房纤维比程度呈密切正相关(r=0.84,P=0.018);血浆血管紧张素Ⅱ、醛固酮以及血清PⅢNP、HA水平较正常对照组均有明显升高(P＜0.05或P＜0.01);LN比较则无统计学差异;血浆血管紧张素Ⅱ水平与醛固酮水平均呈密切正相关(r=0.759,P=0.182).治疗组LVEF较亡I-IF;模型组有明显改善(P＜0.05);持续性AF发生率较CHF模型组有明显降低(P＜G.05);左右心房纤维化程度较CHF模型组均有明显减轻(P＜0.01).结论葛根素注射液联合厄贝沙坦可减轻II-IF时心房纤维比的程度,这可能是其减少CHF时AF发生率及持续时间的机制之一.
목적 관찰갈근소주사액연합액패사탄대심실쾌속기박치충혈성심력쇠갈(congestive heartfailure,CHF)실험견심방전동급심방섬유화적영향.방법 선택건강성년잡충견21지,수궤령위정상조대조조、CI-IF모형조화TMP치료조.채용우심실쾌속기박건립실험견CHF모형.Butst자격유발심방전동(atrial fibrill;tripn,LVEF).초성심동도의검측실험견좌심실사혈분수(1eft ventricular ejection fraction,LVEF).Mallory's삼색법염색검측심방조직섬유비정도.채용방사면역법측정혈장혈관긴장소1[화철고동적농도,측정혈청m형전지원안기말단태(amino-terllllrlal1～eptldcoftypeⅢprocollagen,PⅢ NP)、층점련단백(1alTlmln,l.N)화투명질산(hyaltlronlcacid,HA)적수평.결과 CHF모형조LVEF교정상대조조명현하강(P＜0.01);AF발생솔、지속성AF발생솔급AF지속시간교정상대조조균명현증가(P＜0.01);좌우심방섬유화정도교정상대조조역유명현증가(P＜0.01);AF지속시간여좌심방섬유비정도정밀절정상관(r=0.84,P=0.018);혈장혈관긴장소Ⅱ、철고동이급혈청PⅢNP、HA수평교정상대조조균유명현승고(P＜0.05혹P＜0.01);LN비교칙무통계학차이;혈장혈관긴장소Ⅱ수평여철고동수평균정밀절정상관(r=0.759,P=0.182).치료조LVEF교망I-IF;모형조유명현개선(P＜0.05);지속성AF발생솔교CHF모형조유명현강저(P＜G.05);좌우심방섬유화정도교CHF모형조균유명현감경(P＜0.01).결론 갈근소주사액연합액패사탄가감경II-IF시심방섬유비적정도,저가능시기감소CHF시AF발생솔급지속시간적궤제지일.
Objective To observe the combined effects of puerarin injection and irbesartan on congestive heartfailure(CHF) in dogs with ventricula fibrous ventricular rapid pacing heart failure.Methods 21 healthy mongrel dogs were randomly divided into normal control group,CI-IF model group and the TMP treated group.CHF was established by right ventricular pacing model of canine.Butst was used to evoke atrial fibrillation (atrial fibrill;tripn,LVEF).Echocardiography was used to detect left ventricular ejection fraction in dogs (Left ventricular ejection fraction,LVEF).Mallory's three-color staining was used to detect the degree of atrial fiber.The level of plasma angiotensin 1,aldosterone concentration,m-type forelimb of the original N-terminal peptide (amino-terllllrlal1 ～ eptldcoftype Ⅲ procollagen,P Ⅲ NP),laminin (1 alTlmln,IN),and hyaluronic acid (hyaltlronlcacid,HA)was detected with radioimmunoassay.Results The LVEF of CHF model group decreased significantly compared with the control group(P ＜0.01).AF incidence ,the incidence of persistent AF and AF duration were significantly increased when compared with the control group(P ＜ 0.01).Left atrial fibrosis were also significantly increased in CHF model group than normal control group (P ＜ 0.01).AF duration was positively correlated with the degree of left atrial fiber (r = 0.84,P = 0.018).plasma angiotensin Ⅱ ,aldosterone,and serurn P Ⅲ NP,HA levels were significantly higher than normal control group(P ＜0.05 or P ＜0.01).There was no statistical difference in LN.Plasma angiotensin Ⅱ and aldosterone levels showed a close positive correlation(r =0.759 ,P =0.182).There is significant improvement in LVEF in treatment group when compared with model group(P ＜ 0.05).The incidence of persistent AF was significantly lower in treated group when compared with CHF model(P ＜ 0.05).The left atrial fibrosis was significantly reduced in treated group when compared with CHF(P ＜ 0.01).Conclusion Puerarin injection combined with irbesartan can reduce II-IF degree of atrial fiber,which may be one of the mechanisms of the reduction of the incidence and duration of CHF and AF.