CAJ | 학술논문

目的调查甘肃省迭部和铧尖地区4种蜱自然感染莱姆病伯氏疏螺旋体(Bb)情况，为莱姆病的防治提供科学依据。方法 2010年3月至6月，在甘肃省迭部和铧尖地区岷山北麓迭部林区（秦岭山脉）和肃南祁连山北麓铧尖林区，对4种优势蜱（森林革蜱、草原革蜱、日本血蜱和青海血蜱）自然感染莱姆病Bb进行流行病学检测。采用夹夜法，每隔10 m布夹，晚放晨收，将捕获的啮齿类动物逆毛检虫法采集寄生蜱，同时采用拖旗法采集游离蜱。对采集的4种活的成蜱，清洗消毒后解剖取其中肠内容物分别涂片，置暗视野镜下观察莱姆病Bb;对所分离到的螺旋体再用Bb单克隆和多克隆抗体鉴别试验加以证实。结果共采集到蜱类2科8属36种，即硬蜱科6属33种，软蜱科2属3种。解剖森林革蜱、草原革蜱、日本血蜱和草原硬蜱4种201只蜱的肠道，暗视野观察出携带莱姆病Bb的阳性蜱25只，阳性率为12.44％(25/201)；接种培养森林革蜱、草原革蜱和日本血蜱3种65只蜱，从12只蜱体内培养分离出莱姆病Bb,阳性率为18.46％(12/65)。结论森林革蜱、草原革蜱和日本血蜱均有程度不同地莱姆病Bb的自然感染。
목적 조사감숙성질부화화첨지구4충비자연감염래모병백씨소라선체(Bb)정황，위래모병적방치제공과학의거。방법 2010년3월지6월，재감숙성질부화화첨지구민산북록질부림구（진령산맥）화숙남기련산북록화첨림구，대4충우세비（삼림혁비、초원혁비、일본혈비화청해혈비）자연감염래모병Bb진행류행병학검측。채용협야법，매격10 m포협，만방신수，장포획적교치류동물역모검충법채집기생비，동시채용타기법채집유리비。대채집적4충활적성비，청세소독후해부취기중장내용물분별도편，치암시야경하관찰래모병Bb;대소분리도적라선체재용Bb단극륭화다극륭항체감별시험가이증실。결과 공채집도비류2과8속36충，즉경비과6속33충，연비과2속3충。해부삼림혁비、초원혁비、일본혈비화초원경비4충201지비적장도，암시야관찰출휴대래모병Bb적양성비25지，양성솔위12.44％(25/201)；접충배양삼림혁비、초원혁비화일본혈비3충65지비，종12지비체내배양분리출래모병Bb,양성솔위18.46％(12/65)。결론 삼림혁비、초원혁비화일본혈비균유정도불동지래모병Bb적자연감염。
Objective In order to offer scientific evidence for prevention and therapy of Lyme disease, we had investigated the natural infection of lyme disease Borrelia burgdorferi(Bb) of 4 ticks in Diebu and Huajian areas of Gansu province. Methods Epidemiology detection of natural infection of lyme disease Bb was accomplished for four dominant tick ( Dermacetor silvarum , Dermacentor nuttalli , Haemaphysalis Japonica and Ixodes Crenulatus ) at Diebu, Diebu forest zone(Qin mountain), north of Min Mountains of Huajian region and Huajian forest zone, north of Qilian Mountains of Sunan region, Gansu, from March to June, 2010. The methods of dispersion of clamp every 10 m which was put in the morning and retrieved at night were used to capture rodent animal and gnawer retrorse hair inspection insect methods were used to collect parasitic tick. Flagging methods were used to collect free tick.Four kinds of live adult ticks were dissected after cleaning and disinfection. Intestinal contents were smeared, lyme disease Bb was observed under dark-field microscope. Etiological agent was cultivated and separated. Separated spirochete was confirmed by spirochete monoclonal and polyclonal antibody. Results The ticks collected were classified as 2 families, 8 genera, 36 species, i.e. Ixodidae 6 genera, 33 species and Argasidae 2 genera, 3 species. The method of dark-field microscope was used to detect the lyme disease spirochete in 201 ticks intestines after dissection of Dermacentor silvarum, Dermacentor nuttalli, Haemaphysalis Japonica and Ixodes Crenulatus, the lyme disease Bb-positive were 25 with positive rate of 12.44％(25/201). The lyme disease Bb was cultivated and separated from 12 ticks with the positive rate of 18.46％(12/65) after inoculation and cultivation of 3 species, 65 ticks of Dermacentor silvarum, Dermacentor nuttalli and Haemaphysalis Japonica. Conclusions Dermacentor silvarum,Dermacentor nuttalli and Haemaphysalis Japonica have different degrees of natural infection of lyme disease Bb.