CAJ | 학술논문

目的：优化金银花样品中绿原酸和木犀草苷提取工艺，并建立两者含量同时测定的方法。方法：设计三水平四因素的正交试验，筛选最佳提取条件，并以此条件处理不同产地的金银花，用HPLC测定其绿原酸和木犀草苷的含量。结果：最佳提取工艺条件为：用50％的乙醇35mL在70℃下回流提取1h；0．5％冰醋酸和甲醇为流动相，梯度洗脱，检测波长350nm，绿原酸和木犀草苷的线性方程Y=1×106x-8067和Y=4×106X-119017，线性区间为：0．2-1．6ug和O．22-2．21ug，回归系数r值为0。9999和0．9997；精密度、稳定性、回收率试验结果均良好；五个产地的金银花有四个绿原酸和木犀草苷的含量高于药典规定。
목적：우화금은화양품중록원산화목서초감제취공예，병건립량자함량동시측정적방법。방법：설계삼수평사인소적정교시험，사선최가제취조건，병이차조건처리불동산지적금은화，용HPLC측정기록원산화목서초감적함량。결과：최가제취공예조건위：용50％적을순35mL재70℃하회류제취1h；0．5％빙작산화갑순위류동상，제도세탈，검측파장350nm，록원산화목서초감적선성방정Y=1×106x-8067화Y=4×106X-119017，선성구간위：0．2-1．6ug화O．22-2．21ug，회귀계수r치위0。9999화0．9997；정밀도、은정성、회수솔시험결과균량호；오개산지적금은화유사개록원산화목서초감적함량고우약전규정。
Objective： To optimalize the extracting conditions of chlorogenic acid and galuteolin from Flos lonicerae, and develop one method for the content determination of chlorogenic acid and galuteohn. Methods： Through designing a L9 （34） orthogonal test to screen the best extracting parameters and extract chlorogenic acid and galuteolin in Flos lonicerae of the five different production regions under the condition. Then a HPLC method was dopted to determine the content of those samples. Results： Orthogonal experiment demonstrated that the best extracting conditions included solution of 35mL 50% ethanol and extracting time of one hour at 70~C. HPLC method： the mobile phase was 0.5% acetic acid and methanol, gradient elution separation,and detect at wave- length 350nm. A good linearity of chlorogenic acid and galuteolin was within the range of 0.2 - 1.6 ug and 0.2 -2.2 ug respectively, and the linear equations was y = 1 x 106x - 806 7 and y = 4 x 106x - 119 017, with regression coefficient 0. 999 9 and 0. 999 7. The precision, reproducibility, recovery of the method were all proved perfect. The four content of the chlorogenic acid and galuteolin of the five different production regions are higher than the required standard of Chinese Pharmacopoeia.