CAJ | 학술논문

本文介绍了一种简单的显性标记,可辅助香稻选育.该方法根据大部分香稻的BADH2第7外显子的8碱基差异,分别设计香稻特异引物Frg-Y,和非香稻特异引物的Frg-N,再设计一条公用引物Frg-share.利用Frg-Y和Frg-share即可筛选含有香味基凶的材料;利用Frg-N和Frg-share可辅助鉴定香味基因是否纯合.扩增结果可以在琼脂胶上显示,并且由于带型简单,可以多次点样,非常适于育种过程中的大量样品筛选.
본문개소료일충간단적현성표기,가보조향도선육.해방법근거대부분향도적BADH2제7외현자적8감기차이,분별설계향도특이인물Frg-Y,화비향도특이인물적Frg-N,재설계일조공용인물Frg-share.이용Frg-Y화Frg-share즉가사선함유향미기흉적재료;이용Frg-N화Frg-share가보조감정향미기인시부순합.확증결과가이재경지효상현시,병차유우대형간단,가이다차점양,비상괄우육충과정중적대량양품사선.
An 8 bp deletion in the exon7 of BA DH2(betaine aldehyde dehydrogenase homologue 21 gene results in the fragrance of rice and most fragrant rice contain such deleted-BA DH2 gene.Although some markers targeting the InDel polymorphism have been reported for genotyping fragrance trait,they were found to have limitations or disadvantages.In order to accelerate the fragrant rice breeding,we have developed all agarose-gel-based,two-tube dominant marker which targets the allelic difference of BADH2.The primer Frg-Y binds specifically to the deleted-exon7 of BA DH2 and pfizer Frg-N binds specifically to the full-BA DH2.To validate the utility of this method,F2 individuals segregating for flagrance were detected simultaneously with both a classical InDel marker (GRFM04)and this new marker.The same PCR amplification paaem could be obtained through those two markers.The marker Frg-Y(including primers Frg-share and Frg-Y) can be solely used to check ifindividual plants of breeding lines contain the fragrant alleles in regardless of their allelic homozygosity during the intermediate steps of breeding,while the combination of markers Frg-Y and Frg-N Can display the allelic homozygosity of fragrance at the final step of breeding.Besides,as only one band appears on each electrophoresis lane,several batches of DNA samples can be loaded in a 15-minute interval on a same agarose gel,resulting in a big time and cost reduction.