CAJ | 학술논문

经非水反相高效液相色谱(NARP-HPLC)纯化,从癞葡萄籽油(MCVO)中分离得到了6个含共轭亚麻酸(CLns)的甘油三酯(TGs)组分.以纯度为96%的α-桐酸(α-ESA)为对照,考察甘油三酯组分对5个人体肿瘤细胞株(ECA-109、SGC-7901、Bel-7402、LS-174-T、KB)和1个人体正常细胞株(L02)的生长抑制效果.结果显示含CLn的TG组分对人体肿瘤细胞的抑制作用远强于α-ESA,而且由3个CLns组成的TG是TG组分中效果最弱的.结果表明:TG的脂肪酸组成和TG的分子结构(可使CLn稳定性提高)对含CLn的TG组分抑制人体肿瘤细胞的生长具有重要影响.
경비수반상고효액상색보(NARP-HPLC)순화,종라포도자유(MCVO)중분리득도료6개함공액아마산(CLns)적감유삼지(TGs)조분.이순도위96%적α-동산(α-ESA)위대조,고찰감유삼지조분대5개인체종류세포주(ECA-109、SGC-7901、Bel-7402、LS-174-T、KB)화1개인체정상세포주(L02)적생장억제효과.결과현시함CLn적TG조분대인체종류세포적억제작용원강우α-ESA,이차유3개CLns조성적TG시TG조분중효과최약적.결과표명:TG적지방산조성화TG적분자결구(가사CLn은정성제고)대함CLn적TG조분억제인체종류세포적생장구유중요영향.
Six fractions of triacylglycerols (TGs) containing conjugated linolenic acids (CLns) were separated from the seed oil of Momordica charantia L. vat. abbreviata Set. (MCVO) and purified by non-aqucous reversed-phase (NARP)-HPLC. The cytotoxic effects of the fractions were further investigated on five human tumor cell lines: ECA-109 (esophageal carcinoma),SGC-7901 (carcinoma ventficuli), Bel-7402 (hepatoma), LS-174-T (colon carcinoma), KB (mouth epidermal carcinoma), and a human normal cell line: L02 (liver cell), α-Eleostearic acid (α-ESA) with a purity of 96% was used as a control. The results demonstrated that the inhibitory effects of the TG fractions on human tumor cell lines were more potent than that of α-ESA,and the effect of TG with three CLns was the weakest.These results suggest that the fatty acid compositions and molecularstructures of the TG with more intense stability, which contain CLn, might affect the displayed cytotoxicity to human tumor cell lines.