中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
CHINESE JOURNAL OF BIOCHEMICAL PHARMACEUTICS
2010年
2期
77-81
,共5页
李恒%和七一%余晓东%柳建平%宋锡迅%文浩平%邓疆渝
李恆%和七一%餘曉東%柳建平%宋錫迅%文浩平%鄧疆渝
리항%화칠일%여효동%류건평%송석신%문호평%산강투
白唇竹叶青蛇%5'-核苷酸酶%蛇毒%血小板聚集
白脣竹葉青蛇%5'-覈苷痠酶%蛇毒%血小闆聚集
백진죽협청사%5'-핵감산매%사독%혈소판취집
Trimeresurus albolabris%5'-nucleotidase%snake venom%platelet aggregation
目的 研究白唇竹叶青蛇毒5'-核苷酸酶对血小板聚集的影响,探讨其作用机制.方法 采用比浊法测定白唇竹叶青蛇毒5'-核甘酸酶对二磷酸腺苷(ADP)、花生四烯酸(AA)、血小板活化因子(PAF)诱导血小板聚集的影响.结果 白唇竹叶青蛇毒5'-核甘酸酶能抑制ADP、AA、PAF诱导的血小板聚集,且其抑制率与剂量增加成正比;抑制血小板聚集作用与ADP的水解和腺苷的积累有关.结论 白唇竹叶青蛇毒5'-核甘酸酶能抑制血小板聚集,抑制作用主要通过水解ADP和积累腺苷,可能与阻断僦的生成有关.
目的 研究白脣竹葉青蛇毒5'-覈苷痠酶對血小闆聚集的影響,探討其作用機製.方法 採用比濁法測定白脣竹葉青蛇毒5'-覈甘痠酶對二燐痠腺苷(ADP)、花生四烯痠(AA)、血小闆活化因子(PAF)誘導血小闆聚集的影響.結果 白脣竹葉青蛇毒5'-覈甘痠酶能抑製ADP、AA、PAF誘導的血小闆聚集,且其抑製率與劑量增加成正比;抑製血小闆聚集作用與ADP的水解和腺苷的積纍有關.結論 白脣竹葉青蛇毒5'-覈甘痠酶能抑製血小闆聚集,抑製作用主要通過水解ADP和積纍腺苷,可能與阻斷僦的生成有關.
목적 연구백진죽협청사독5'-핵감산매대혈소판취집적영향,탐토기작용궤제.방법 채용비탁법측정백진죽협청사독5'-핵감산매대이린산선감(ADP)、화생사희산(AA)、혈소판활화인자(PAF)유도혈소판취집적영향.결과 백진죽협청사독5'-핵감산매능억제ADP、AA、PAF유도적혈소판취집,차기억제솔여제량증가성정비;억제혈소판취집작용여ADP적수해화선감적적루유관.결론 백진죽협청사독5'-핵감산매능억제혈소판취집,억제작용주요통과수해ADP화적루선감,가능여조단추적생성유관.
Purpose To investigate the effect of 5'-nucleotidase from Trimeresurus albolabris venom on platelet aggregations and its mechanism.Methods Piatelet aggregations induced by ADP,AA and PAF,respectively.were measured by turbidimetric method after platelet incubated with 5'-nucleotidase.Results 5'-nucleotidase significantly inhibited platelet aggregations induced by ADP,AA and PAF in a dose-dependent manner.Washed platelet inhibition experiment and the effect of Adenosine,CP/CPK on platelet aggregations showed that platelet aggregations inhibited by 5'-nucleotidase were associated with ADP hydrolysis and adenosine accumulation.The effect of ASA on platelet aggregations showed that the enzyme inhibited platelet aggregations probably by blocking the formation of TXA2.Conclusion 5'-nucleotidase from Trimeresurus albolabris venom inhibited platelet aggregations through ADP hydrolysis and adenosine accumulation,and maybe it is related to blocking the formation of TXA2.
