CAJ | 학술논문

目的探讨儿茶素对大鼠脑缺血/再灌注损伤的保护作用及机制.方法 40只大鼠随机分成5组,假手术组、模型组和儿茶素低、中、高剂量(50,100,200 mg·kg~(-1))组.采用改良的线栓法制备大鼠局灶性脑缺血(2 h)/再灌注(24 h)损伤模型,儿茶素各组于缺血前30 min和缺血后2 h腹腔注射儿茶素溶液,假手术组和模型组给与等体积的NS.再灌注24 h,用改良的Bederson评分法对大鼠进行神经功能缺损评分后,进行海马组织形态学观察及MPO和NOS活性测定.结果儿茶素可降低大鼠脑缺血/再灌注损伤的神经功能缺损评分,能不同程度地改善脑组织学损伤,并且降低脑组织中MPO和NOS的活性.结论儿茶素对大鼠脑缺血/再灌注损伤有保护作用,作用机制之一可能与抗炎、抗氧化有关.
목적 탐토인다소대대서뇌결혈/재관주손상적보호작용급궤제.방법 40지대서수궤분성5조,가수술조、모형조화인다소저、중、고제량(50,100,200 mg·kg~(-1))조.채용개량적선전법제비대서국조성뇌결혈(2 h)/재관주(24 h)손상모형,인다소각조우결혈전30 min화결혈후2 h복강주사인다소용액,가수술조화모형조급여등체적적NS.재관주24 h,용개량적Bederson평분법대대서진행신경공능결손평분후,진행해마조직형태학관찰급MPO화NOS활성측정.결과 인다소가강저대서뇌결혈/재관주손상적신경공능결손평분,능불동정도지개선뇌조직학손상,병차강저뇌조직중MPO화NOS적활성.결론 인다소대대서뇌결혈/재관주손상유보호작용,작용궤제지일가능여항염、항양화유관.
Aim To investigate the protective effect of catechin on cerebral ischemia-reperfusion injury in rats and its mechanism.Methods 40 rats were randomly divided into 5 groups:sham operation group,model group and 50,100 and 200 mg·kg~(-1) catechin groups,with 8 rats in each group.The model of focal cerebral ischemia-reperfusion in rats was established with modified sutured-occluded method.The rats in catechin groups were injected with catechin at the matched concentration.The rats in sham operation group and model group were injected with saline.And all rats were given more time in 2 hours after ischemia.Rats were sacrificed for histologic examination after the behavioral test,and their brains were taken to assay the activities of MPO and NOS.Results Catechin at different dosages(50,100 and 200 mg·kg~(-1))could obviously decrease neurological deficit score,repair histological injury,and reduce the activities of MPO and NOS in rats of focal cerebral ischemia-reperfusion injury.Conclusions Catechin can relieve the cerebral ischemia reperfusion injury,and its mechanism may be partly related to the effects of its antiinflammation and antioxidation.