CAJ | 학술논문

目的探讨超声靶向破坏超声微泡介导脂质体小干扰RNA(siRNA)转染视网膜色素上皮(RPE)细胞的价值. 方法将人及大鼠RPE细胞隔孔接种于24孔板中(2×10~5/孔、1×10~5/孔),分5组处理:siRNA+超声(US)、siRNA+微泡(MBs)+US、siRNA+脂质体(L)、siRNA+L+US、siRNA+L+MBs+US.12小时后,荧光显微镜观察并应用流式细胞仪测定阳性细胞比例. 结果在无脂质体的条件下,超声或超声联合微泡不能促进siRNA转染人及大鼠RPE细胞.siRNA+L+US组siRNA转染人RPE细胞效率最高.siRNA+L+US+MB组siRNA转染人及大鼠RPE细胞的效率显著低于siRNA+L+US组. 结论超声辐照可促进脂质体介导的siRNA转染人RPE细胞.
목적 탐토초성파향파배초성미포개도지질체소간우RNA(siRNA)전염시망막색소상피(RPE)세포적개치. 방법장인급대서RPE세포격공접충우24공판중(2×10~5/공、1×10~5/공),분5조처리:siRNA+초성(US)、siRNA+미포(MBs)+US、siRNA+지질체(L)、siRNA+L+US、siRNA+L+MBs+US.12소시후,형광현미경관찰병응용류식세포의측정양성세포비례. 결과재무지질체적조건하,초성혹초성연합미포불능촉진siRNA전염인급대서RPE세포.siRNA+L+US조siRNA전염인RPE세포효솔최고.siRNA+L+US+MB조siRNA전염인급대서RPE세포적효솔현저저우siRNA+L+US조. 결론초성복조가촉진지질체개도적siRNA전염인RPE세포.
Objective To investigate the transfection efficiency of combination of ultrasound (US) microbubbles (MBs) mediated liposome small interference RNA (siRNA) to human and rat retinal pigment epithelium (RPE) cells. Methods Human and rat RPE cells and siRNA were incubated in 24-well plates (2×10~5/well and 1×10~5/well, respectively). The cells were devided into 5 groups: siRNA+US, siRNA+MBs+US, siRNA+L (liposome), siRNA+L+US, siRNA+L+US+MBs. After 12 h, transfection efficiency was observed with fluorescence microscopy and flow cytometry. Results US or ultrasound targeted microbubbles destruction without liposome-mediated could not promote siRNA transfection efficiency to human and rat RPE cells. The transfection efficiency of human and rat RPE cells significantly decreased in the siRNA+L+US+MBs group, but increased in siRNA+L+US group. Conclusion Ultrasonic irradiation can promote lipid-mediated siRNA transfected human RPE cells.