CAJ | 학술논문

从147株南极海冰细菌中筛选出一株高产低温α-淀粉酶的菌株Pseudoalteromonas sp. AN175.生长发酵单因素试验表明,菌株AN175在1%麦芽糖、0.5 %酵母粉、0.01%磷酸铁、0.5%氯化钙、1%可溶性淀粉、5% NaCl、pH 6.0的培养基,10 ℃,30%装液量,130 r/min条件下振荡培养9 d可达到最大酶活132.5 U/mL,为优化前的2倍多.生长和产酶试验表明,该菌生长较常温菌缓慢,酶分泌时间滞后.粗酶特性研究显示,该酶催化的最适条件为温度30 ℃,pH值8.0.
종147주남겁해빙세균중사선출일주고산저온α-정분매적균주Pseudoalteromonas sp. AN175.생장발효단인소시험표명,균주AN175재1%맥아당、0.5 %효모분、0.01%린산철、0.5%록화개、1%가용성정분、5% NaCl、pH 6.0적배양기,10 ℃,30%장액량,130 r/min조건하진탕배양9 d가체도최대매활132.5 U/mL,위우화전적2배다.생장화산매시험표명,해균생장교상온균완만,매분비시간체후.조매특성연구현시,해매최화적최괄조건위온도30 ℃,pH치8.0.
The antarctic strain Pseudoalteromonas sp. AN175 was screened from 147 sea-ice bacteria which could produce low-temperature α-amylase. Through single factor method experiment, the enzyme-producing conditions were 1% maltose, 0.5% yeast powder, 0.01% Fe_3PO_4, 0.5% CaCl_2, 1% soluble starch, 5% NaCl, pH 6.0, at 10 ℃, 130 r/min shaking bed cultivated for 9 days. Under optimal cultivate conditions, α-amylase activity was improved from 64.5 U/mL to 132.5 U/mL. Comparing to temperature bacteria strain, sea-ice strain AN175 grew and produced enzyme slower. Preliminary studies showed that the most adaptable reaction temperature and pH for α-amylase were 30 ℃ and 8.0 respectively.