中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
CHINESE JOURNAL OF BIOCHEMICAL PHARMACEUTICS
2009年
6期
368-370,374
,共4页
柴瑜%罗欣%黄海良%胡道军%陶自泽%张胜权
柴瑜%囉訢%黃海良%鬍道軍%陶自澤%張勝權
시유%라흔%황해량%호도군%도자택%장성권
白介素29%乙肝病毒%HepG2.2.15细胞
白介素29%乙肝病毒%HepG2.2.15細胞
백개소29%을간병독%HepG2.2.15세포
IL-29%hepatitis B virus%HepG2.2.15 cell
目的 研究白介素29(IL-29)体外抗乙肝病毒的效果.方法 从RNA水平探讨了IL-29抗乙肝病毒的效果.RT-PCR分析IL-29诱导抗病毒蛋白MxA、2′,5′-OAS、PKR和RNase L mRNA水平表达;同时Western blot分析IL-29激活的信号通路以探讨其抗乙肝病毒的机理.结果 IL-29能明显降低HepG2.2.15细胞中乙肝病毒mRNA的水平,且能够上调抗病毒蛋白MxA和2′,5′-OAS的mRNA表达并激活ERK1/2、AKT信号途径.结论 在HepG2.2.15细胞中IL-29有显著的抗乙肝病毒作用.
目的 研究白介素29(IL-29)體外抗乙肝病毒的效果.方法 從RNA水平探討瞭IL-29抗乙肝病毒的效果.RT-PCR分析IL-29誘導抗病毒蛋白MxA、2′,5′-OAS、PKR和RNase L mRNA水平錶達;同時Western blot分析IL-29激活的信號通路以探討其抗乙肝病毒的機理.結果 IL-29能明顯降低HepG2.2.15細胞中乙肝病毒mRNA的水平,且能夠上調抗病毒蛋白MxA和2′,5′-OAS的mRNA錶達併激活ERK1/2、AKT信號途徑.結論 在HepG2.2.15細胞中IL-29有顯著的抗乙肝病毒作用.
목적 연구백개소29(IL-29)체외항을간병독적효과.방법 종RNA수평탐토료IL-29항을간병독적효과.RT-PCR분석IL-29유도항병독단백MxA、2′,5′-OAS、PKR화RNase L mRNA수평표체;동시Western blot분석IL-29격활적신호통로이탐토기항을간병독적궤리.결과 IL-29능명현강저HepG2.2.15세포중을간병독mRNA적수평,차능구상조항병독단백MxA화2′,5′-OAS적mRNA표체병격활ERK1/2、AKT신호도경.결론 재HepG2.2.15세포중IL-29유현저적항을간병독작용.
Purpose To explore the antiviral effect of interleukin 29(IL-29) on hepatitis B virus in vitro.Methods To study the antiviral effect of IL-29 against hepatitis B virus by the amount of HBV mRNA detected .Through the quantity of mRNA translated from genes of MxA,2′,5′-OAS,PKR and RNase L as well as the signal pathway induced by IL-29,we used RT-PCR and Western blot to discuss the anti-hepatitis B virus mechanism which was stimulated by IL-29.Results The amount of HBV mRNA in HepG2.2.15 cells was reduced by stimulation of IL-29.The expression of MxA and 2′,5′-OAS was up-regulated,as well as P-ERK and P-AKT were activated by IL-29.Conclusion These findings showed that IL-29 had obvious antiviral activity towards HBV in HepG2.2.15 cells.
