工业微生物
工業微生物
공업미생물
INDUSTRIAL MICROBIOLOGY
2009年
6期
20-24
,共5页
黄铜矿精矿%槽式搅拌反应器%中度嗜热菌%PCR-RFLP%种群结构
黃銅礦精礦%槽式攪拌反應器%中度嗜熱菌%PCR-RFLP%種群結構
황동광정광%조식교반반응기%중도기열균%PCR-RFLP%충군결구
chalcopyrite concentrate%stirred tank bioreactor%moderately thermophilic microorganism%PCR-RFLP%community structure
为了优化浸出工艺,研究了pH对浸矿过程主要微生物种群结构的影响.用中度嗜热混合菌槽浸黄铜矿精矿,在不控制pH,控制pH为2.5及控制pH为1.2时,应用PCR-RFLP(限制性酶切片段长度多态性)方法对上述浸出条件下的细菌群落动态变化进行研究.结果表明,浸出体系只有两种微生物,一种为 Acidithiobacillus caldus,一种为 Leptospirillum ferriphilum.pH对群落结构有明显影响.不控制pH时,浸出开始阶段At.caldus 是优势种群,占群落的96%,随着浸出的进行,L.ferriphilum增多,在浸出后期代替At.caldus成为优势菌种,占69%.控制pH时,L.ferriphilum 始终占主导地位,同时发现pH为2.5时At.caldus 在群落中的丰度比pH为1.2时高.
為瞭優化浸齣工藝,研究瞭pH對浸礦過程主要微生物種群結構的影響.用中度嗜熱混閤菌槽浸黃銅礦精礦,在不控製pH,控製pH為2.5及控製pH為1.2時,應用PCR-RFLP(限製性酶切片段長度多態性)方法對上述浸齣條件下的細菌群落動態變化進行研究.結果錶明,浸齣體繫隻有兩種微生物,一種為 Acidithiobacillus caldus,一種為 Leptospirillum ferriphilum.pH對群落結構有明顯影響.不控製pH時,浸齣開始階段At.caldus 是優勢種群,佔群落的96%,隨著浸齣的進行,L.ferriphilum增多,在浸齣後期代替At.caldus成為優勢菌種,佔69%.控製pH時,L.ferriphilum 始終佔主導地位,同時髮現pH為2.5時At.caldus 在群落中的豐度比pH為1.2時高.
위료우화침출공예,연구료pH대침광과정주요미생물충군결구적영향.용중도기열혼합균조침황동광정광,재불공제pH,공제pH위2.5급공제pH위1.2시,응용PCR-RFLP(한제성매절편단장도다태성)방법대상술침출조건하적세균군락동태변화진행연구.결과표명,침출체계지유량충미생물,일충위 Acidithiobacillus caldus,일충위 Leptospirillum ferriphilum.pH대군락결구유명현영향.불공제pH시,침출개시계단At.caldus 시우세충군,점군락적96%,수착침출적진행,L.ferriphilum증다,재침출후기대체At.caldus성위우세균충,점69%.공제pH시,L.ferriphilum 시종점주도지위,동시발현pH위2.5시At.caldus 재군락중적봉도비pH위1.2시고.
In order to investigate influence of pH on microbial population dynamics in bioleaching system for process optimization,chalcopyrite concentrate was leached by moderately thermophilic mixed microorganism in a stirred tank bioreactor.The method of PCR-RFLP was used to analyze the succession of bacterial community under conditions of uncontrolled pH and pH controlled at 2.5 or 1.2.There were only two species,Acidithiobacillus caldus and Leptospirillum ferriphilum.When pH was not controlled,at the beginning of bioleaching process,At.caldus was the predominant species,accounted for 96% of the whole community.With the process of leaching,L.ferriphilum increased and replaced At.caldus,and accounted for 69% in the later stage of bioleaching; When pH was controlled,L.ferriphilum was always predominant species.It was found that abundance of At.caldus in the whole community at pH 2.5 was more than the situation of pH 1.2.Community diversity of bioleaching system in this experiment was simple.pH had obvious influence on microbial population dynamics.
