CAJ | 학술논문

目的观察十全大补汤配方颗粒对骨髓抑制小鼠外周血及促红细胞生成素(EPO)的影响.方法采用60钴(60Co)照射、注射环磷酰胺、氯霉素复合处理建立小鼠骨髓抑制模型后,给予十全大补汤配方颗粒进行治疗,采用全自动血细胞分析仪检测其对外周血的影响;双抗体夹心生物素-亲和素复合物酶联免疫吸附试验(ABC-ELISA)法检测血清中EPO含量;逆转录-聚合酶链反应(RT-PCR)检测骨髓及肾脏EPO mRNA表达情况.结果与正常对照组比较,模型组小鼠外周血血象、骨髓有核细胞数降低,血清EPO含量升高,肾脏、骨髓细胞EPO mRNA表达增强.经十全大补汤配方颗粒治疗后,外周血、骨髓有核细胞数升高,血清EPO含量升高,肾脏、骨髓有核细胞EPO mRNA的表达增强.结论十全大补汤配方颗粒可能通过在转录水平上促进肾脏、骨髓有核细胞EPO mRNA的表达,促进受损骨髓红系造血的恢复.
목적 관찰십전대보탕배방과립대골수억제소서외주혈급촉홍세포생성소(EPO)적영향.방법 채용60고(60Co)조사、주사배린선알、록매소복합처리건립소서골수억제모형후,급여십전대보탕배방과립진행치료,채용전자동혈세포분석의검측기대외주혈적영향;쌍항체협심생물소-친화소복합물매련면역흡부시험(ABC-ELISA)법검측혈청중EPO함량;역전록-취합매련반응(RT-PCR)검측골수급신장EPO mRNA표체정황.결과 여정상대조조비교,모형조소서외주혈혈상、골수유핵세포수강저,혈청EPO함량승고,신장、골수세포EPO mRNA표체증강.경십전대보탕배방과립치료후,외주혈、골수유핵세포수승고,혈청EPO함량승고,신장、골수유핵세포EPO mRNA적표체증강.결론 십전대보탕배방과립가능통과재전록수평상촉진신장、골수유핵세포EPO mRNA적표체,촉진수손골수홍계조혈적회복.
Objective To examine the effect of Shi-Quan-Da-Bu- Granule on routine blood, EPO serum concentration and expression of EPOmRNA in kidney and BMC of myelosuppressed mice.Methods Set up myelosuppressed mice model by means of Co60 irradiation, cyclophosphamide injection, and chlorampHenicol injection. The count of blood cell was determined by blood auto-analyzer. Serum concentration of Epo was measured by ELISA. Expression of EPOmRNA in kidney and BMC were determined by RT-PCR.Results Compared with normal control group, the count of blood cell, the number of BMC decreased, and serum concentration of EPO and expression of EPOmRNA in kidney and BMC increased. All of above indexes in high-dose and mid-dose of Shi-Quan-Da-Bu-Granule treatment group were higher than that of model control group(P＜0.05).Conclusion Shi-Quan-Da- Bu-Granule could promote erythroid hematopoiesis by enhancing the production of EPO and up-regulating expression of EPOmRNA in kidney and BMC of myelosuppression mice.