CAJ | 학술논문

[目的]建立高效的茶菊再生体系和卡那霉素筛选体系.[方法]以茶菊叶盘和茎段为外植体,以MS为基本培养基,通过添加不同浓度的6-BA和NAA设计不同的培养基,研究茶菊叶盘和茎段的最适分化条件,并进行卡那霉素敏感性试验,研究其对不定芽诱导和生根的影响.[结果]茶菊叶盘和茎段直接分化不定芽的最适培养基分别为MS+6-BA 1.0 mg/L+NAA 0.3 mg/L和MS+6-BA 2.0 mg/L+NAA 0.1 mg/L,茶菊生根较容易,在5种培养基上生根率均可达100%;茶菊对卡那霉素反应较为敏感,20.0 mg/L卡那霉素即可抑制叶盘的分化,40.0 mg/L的卡那霉素可抑制茎段的分化,30.0 mg/L卡那霉素可抑制小苗的生根.6 cm左右长的生根无菌苗经炼苗和移栽后成活率为100%.[结论]该试验为茶菊的进一步遗传转化提供了基础.
[목적]건립고효적다국재생체계화잡나매소사선체계.[방법]이다국협반화경단위외식체,이MS위기본배양기,통과첨가불동농도적6-BA화NAA설계불동적배양기,연구다국협반화경단적최괄분화조건,병진행잡나매소민감성시험,연구기대불정아유도화생근적영향.[결과]다국협반화경단직접분화불정아적최괄배양기분별위MS+6-BA 1.0 mg/L+NAA 0.3 mg/L화MS+6-BA 2.0 mg/L+NAA 0.1 mg/L,다국생근교용역,재5충배양기상생근솔균가체100%;다국대잡나매소반응교위민감,20.0 mg/L잡나매소즉가억제협반적분화,40.0 mg/L적잡나매소가억제경단적분화,30.0 mg/L잡나매소가억제소묘적생근.6 cm좌우장적생근무균묘경련묘화이재후성활솔위100%.[결론]해시험위다국적진일보유전전화제공료기출.
[Objective]The study aimed to establish the high efficient regeneration system and screening system to Kanamycin in Chaju. [Method]With leaf discs and stem segments of Chaju as explants, MS was used as the basic medium and it was supplemented with 6-BA and NAA at different concn. for designing different media in order to study the optimum differentiation conditions of leaf discs and stem segments of Chaju. Kanamycin sensitive tests were carried out to study its effect on adventitious bud induction and rooting. [Result]The optimum medium for leaf discs and stem segments of Chaju inducing directly adventitious bud differentiation were MS + 6-BA 1.0 mg/L + NAA 0.3 mg/L and MS + 6-BA 2.0 mg/L + NAA 0.1 mg/L, resp. and rooting of Chaju was more easily on 5 kinds of culture medium with the rooting rate of 100%;Chaju was sensitive to kanamycin, in which, 20.0 mg/L kanamycin could inhibit the differentiation of leaf discs, 40.0 mg/L kanamycin inhibited the differentiation of stem, and 30.0 mg/L Kanamycin inhibited rooting of seedlings. Aseptic seedling about 6 cm in length had survival rate of 100% after hardening and transplanting. [Conclusion]The experiment provided a basis for further genetic transformation of Chaju.