CAJ | 학술논문

目的探讨高糖和氧化型低密度脂蛋白(ox-LDL)对THP-1源性巨噬细胞转化的影响.方法体外培养人THP-1单核细胞系,由佛波酯作用使其转化为巨噬细胞,并随机分为四组(n=6):对照组、高糖组(30 mmol/L葡萄糖)、ox-LDL组(100μg/mLox-LDL)和高糖/ox-LDL(G-ox-LDL)组(30 mmol/L葡萄糖+100 μg/mL ox-LDL).应用高效液相色谱法检测细胞内总胆固醇和胆固醇酯含量;透射电镜和油红O染色光镜观察细胞内脂质.结果 ox-LDL组和G-ox-LDL组细胞胞质内出现大量的红染颗粒或脂质空泡,总胆固醇和胆固醇酯均较对照组增加,差异有统计学意义(P<0.05);且两组胆固醇酯含量均大于总胆固醇含量的50%.对照组和高糖组细胞胞质内可见少量红染颗粒或脂质空泡,两组细胞内总胆固醇和胆固醇酯比较,差异无统计学意义(P>0.05);两组胆固醇酯含量均小于总胆固醇含量的50%.结论 ox-LDL能使THP-1源性巨噬细胞转化泡沫细胞,而高糖不能使THP-1源性巨噬细胞转化泡沫细胞.提示ox-LDL可能是THP-1源性巨噬细胞转化为泡沫细胞的必要条件.
목적 탐토고당화양화형저밀도지단백(ox-LDL)대THP-1원성거서세포전화적영향.방법 체외배양인THP-1단핵세포계,유불파지작용사기전화위거서세포,병수궤분위사조(n=6):대조조、고당조(30 mmol/L포도당)、ox-LDL조(100μg/mLox-LDL)화고당/ox-LDL(G-ox-LDL)조(30 mmol/L포도당+100 μg/mL ox-LDL).응용고효액상색보법검측세포내총담고순화담고순지함량;투사전경화유홍O염색광경관찰세포내지질.결과 ox-LDL조화G-ox-LDL조세포포질내출현대량적홍염과립혹지질공포,총담고순화담고순지균교대조조증가,차이유통계학의의(P<0.05);차량조담고순지함량균대우총담고순함량적50%.대조조화고당조세포포질내가견소량홍염과립혹지질공포,량조세포내총담고순화담고순지비교,차이무통계학의의(P>0.05);량조담고순지함량균소우총담고순함량적50%.결론 ox-LDL능사THP-1원성거서세포전화포말세포,이고당불능사THP-1원성거서세포전화포말세포.제시ox-LDL가능시THP-1원성거서세포전화위포말세포적필요조건.
Objective To explore the effects of hyperglycemia and oxidized low density lipoprotein (ox-LDL) on the differentiation of macrophage derived THP-1 monocytes. Methods THP-1 human monocytic leukemia cell line was cultured in vitro, and the differentiation of THP-1 cells into macrophages was induced by phorbol esters. The macrophages were then incubated with the absence of D-glucose and ox-LDL (control group), 30 mmol/L D-glucose (hyperglycemia group), 100 μg/mL ox-LDL (ox-LDL group) or 30 mmol/L D-glucose and 100 μg/mL ox-LDL(G-ox-LDL group) for 24 h. High performance liquid chromatography was used for qualitative and quantitative analysis of intracellular cholesterol and cholesteryl esters. Both light microscope with red oil O staining technique and transmission electron microscope were employed to observe the morphology of treated and control THP-1 cells. Results A large number of intracellular red oil O stained granules and lipid vacuoles were observed in ox-LDL group and G-ox-LDL group, the contents of total cholesterol and cholesteryl esters were significantly higher than those of control group (P<0.05), and the contents of cholesteryl esters were higher than 50% of total cholesterol in both groups. However, only a few intracellular red oil O stained granules and lipid vacuoles were observed in control group and hyperglycemia group, there was no significant difference in the contents of total cholesterol and choleateryl esters between control group and hyperglycemia group (P>0.05), and the contents of cholesteryl esters were less than 50% of total cholesterol in both groups. Conclusion Foam cells form when THP-1 cells are incubated with ox-LDL, while hyperglycemia alone can not convert THP-1 cells to foam cells, indicating that ox-LDL is necessary for the macrophages derived THP-1 monocytes to turn into foam cells.