稀土学报(英文版)
稀土學報(英文版)
희토학보(영문판)
JOURNAL OF RARE EARTHS
2002年
2期
139-144
,共6页
葛志强%元英进%王艳东%马振毅%胡宗定
葛誌彊%元英進%王豔東%馬振毅%鬍宗定
갈지강%원영진%왕염동%마진의%호종정
rare earths%cerium%apoptosis%Taxus cuspidata%cell suspension culture
The standard detection hallmarks of apoptosis of Taxus cuspidata cells in suspension culture with Ce4+ were studied. The condensation and margination of chromatin were observed under the electron microscopy. DNA fragmentation ranged "DNA ladder" on agarose gel electrophoresis. TdT-mediated dUTP nick end labeling (TUNEL) analysis of the cells reveals that the nuclear DNA strand breaks can be identified by labeling free 3′-OH termini. These results suggest that Ce4+ can induce apoptosis of Taxus cuspidata cells and also indicate that there is a certain relationship between apoptosis and secondary metabolite product-Taxol.
