CAJ | 학술논문

对GPI-1046是否具有神经营养作用目前有两种不同的认识.Steiner等认为GPI-1046能促进体外培养的感觉神经节神经元突起生长.但Harper等却没能证明GPI-1046有这样的作用.由于GPI-1046在临床上具有重要应用价值和前景,我们重新评价了GPI-1046对体外培养鸡胚神经节的神经营养作用,发现在低浓度神经生长因子(nerve growth factor,NGF)存在下,GPI-1046能明显促进鸡背根神经节神经突起的生长.
대GPI-1046시부구유신경영양작용목전유량충불동적인식.Steiner등인위GPI-1046능촉진체외배양적감각신경절신경원돌기생장.단Harper등각몰능증명GPI-1046유저양적작용.유우GPI-1046재림상상구유중요응용개치화전경,아문중신평개료GPI-1046대체외배양계배신경절적신경영양작용,발현재저농도신경생장인자(nerve growth factor,NGF)존재하,GPI-1046능명현촉진계배근신경절신경돌기적생장.
The purpose of this investigation was to re-evaluate the neurotrophic effect of GPI-1046 on neurite outgrowth in vitro.GPI-1046 was synthesized and identified with mass spectrometry,nuclear magnetic resonance and elemental analysis.Chicken dorsal root ganglions(DRGs) were removed and divided into three groups:(1)The DRGs were cultured in DMEM containing different concentrations of GPI-1046;(2)The DRGs were cultured in DMEM containing nerve growth factor (NGF)alone at 0.8 and 8 ng/mL,respectively;(3)The DRGs were cultured in DMEM containing both different concentrations of GPI-1046 and NGF at 0.8ng/mL.The results showed that GPI-1046 alone could not stimulate chicken DRG neurite outgrowth;however,GPI-1046 stimulated DRG neurite outgrowth only in the presence of NGF at low concentration in the culture medium.