动物细胞系的染色体组型与遗传变异率分析
동물세포계적염색체조형여유전변이솔분석
Analyses of Chromosomal Karyotypes and Cytogenetic Variations of Animal Cell Lines
  • 万方数据
    遗传学报 유전학보
    ACTA GENETICA SINICA
    2001年 4期 327-344 ,共18页

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    장덕례%리륙금%하경전%하욱옥%고보선%백효홍%황고승%류상고%염륭비%방복덕%호춘뢰%왕려군%강환굉%풍아만%장엄민%안사강%임원강%곽건민%번군희%호숙현%우영리%송점군%리영%번성군

    动物肾细胞系(F-81、CRFK、MDCK、Vero、Vero-2、MA-104、BHK-21)%人类子宫颈癌细胞系(HeLa)%猫肾原代细胞(FKC)%染色体核型%G带核型%遗传变异率%数目变异%结构畸变 動物腎細胞繫(F-81、CRFK、MDCK、Vero、Vero-2、MA-104、BHK-21)%人類子宮頸癌細胞繫(HeLa)%貓腎原代細胞(FKC)%染色體覈型%G帶覈型%遺傳變異率%數目變異%結構畸變
    동물신세포계(F-81、CRFK、MDCK、Vero、Vero-2、MA-104、BHK-21)%인류자궁경암세포계(HeLa)%묘신원대세포(FKC)%염색체핵형%G대핵형%유전변이솔%수목변이%결구기변
    在建立国内首家犬、猫、猴、鼠传代细胞库，即7种动物肾细胞系(F-81、CRFK、MDCK、Vero、Vero-2、MA-104、BHK-21)的种子库和工作库的基础上，通过细胞染色体组型、G带核型、染色体数目变异率、结构畸变率分析，了解7种细胞系传代培养不同代次的染色体变异情况。以相应的细胞株皮下接种裸鼠形成肿瘤实验、软琼脂细胞克隆形成率与植物凝集素作用下细胞凝集实验为对照，筛选出无致癌/致瘤性、符合细胞遗传学要求、无传染因子污染的细胞系(F-81，CRFK、Vero、Vero-2)或极低致癌性的MDCK细胞系用于制苗，发现肿瘤细胞系高变异率株可在裸鼠体内快速选育成功。细胞系染色体遗传特征决定致瘤性质并具有种属特异性，得到一些100％成瘤和100％不成瘤的细胞株并探讨了与染色体组型的关系。对于肿瘤的发病机理及实验治疗，都是非常好的模型。一些细胞系不仅成瘤而且还可转移(致恶性横纹肌样瘤的BHK-21和Vero细胞株)，其他致瘤细胞株只成瘤不转移或不明显转移。
    재건입국내수가견、묘、후、서전대세포고，즉7충동물신세포계(F-81、CRFK、MDCK、Vero、Vero-2、MA-104、BHK-21)적충자고화공작고적기출상，통과세포염색체조형、G대핵형、염색체수목변이솔、결구기변솔분석，료해7충세포계전대배양불동대차적염색체변이정황。이상응적세포주피하접충라서형성종류실험、연경지세포극륭형성솔여식물응집소작용하세포응집실험위대조，사선출무치암/치류성、부합세포유전학요구、무전염인자오염적세포계(F-81，CRFK、Vero、Vero-2)혹겁저치암성적MDCK세포계용우제묘，발현종류세포계고변이솔주가재라서체내쾌속선육성공。세포계염색체유전특정결정치류성질병구유충속특이성，득도일사100％성류화100％불성류적세포주병탐토료여염색체조형적관계。대우종류적발병궤리급실험치료，도시비상호적모형。일사세포계불부성류이차환가전이(치악성횡문기양류적BHK-21화Vero세포주)，기타치류세포주지성류불전이혹불명현전이。
    After the master cell stock(mcs) and working cell bank of more than 30 different strains of 7 animal kidney cell lines (F-81 or CRFK cell line, MDCK cell line, Vero or Vero-2 cell line, MA-104 cell line and BHK-21 cell line) were established in China, the chromosomal number variations and structural aberrations of the above lines, primary feline or canine kidney cell (FKC or CKC) and HeLa cell line were investigated and their karyotypes of routine or Giemsa chromosomal bands were analyzed. The carcinogenesis or tumorigenicity testing of these cells in about 700 nude mice and for colony formation in soft agar (SA) and for agglutination under different concentration of plant lectins was carried out. Both tumorigenicity-negative strains of F-81, CRFK, Vero or Vero-2 lines and very-low-tumorigenicity strains of MDCK line were successfully selected and evaluated for the production of canine or feline combination viral vaccines, which are free of infectious agents, and described with respect to cytogenetic characteristics and tumorigenicity.
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