CAJ | 학술논문

采用激励光源为82MHz、514.5nm的皮秒荧光光谱装置对PSⅡ颗粒、内周天线CP43、CP47三种样品进行研究，通过探测三种样品的荧光总光谱强度随激光功率的变化，测得PSⅡ颗粒样品在激光功率为120mW时，荧光强度趋于饱和；CP43在激光功率为73mW时，荧光趋于饱和，但当激光功率为82mW时，荧光强度有下降趋势；而在激光功率为20～96mW的范围内，CP47的荧光强度与激光功率几乎是线性关系.依据它们的荧光量子产额与激光功率的关系，认为CP47中存在较强的激子效应.几种样品的荧光光谱范围分别为700～780nm(PSⅡ颗粒)；640～780nm(CP43)；630～775nm(CP47).CP43和CP47的最大荧光峰分别为680nm和690nm，荧光寿命分别为3.543ns和3.222ns.在514.5nm激光激发下，CP43和CP47中最先受激发的是β-Car分子，发射荧光的是Chla分子，理论计算认为在CP43和CP47中Chla分子发射荧光的效率分别为38.3%和40.6%.
채용격려광원위82MHz、514.5nm적피초형광광보장치대PSⅡ과립、내주천선CP43、CP47삼충양품진행연구，통과탐측삼충양품적형광총광보강도수격광공솔적변화，측득PSⅡ과립양품재격광공솔위120mW시，형광강도추우포화；CP43재격광공솔위73mW시，형광추우포화，단당격광공솔위82mW시，형광강도유하강추세；이재격광공솔위20～96mW적범위내，CP47적형광강도여격광공솔궤호시선성관계.의거타문적형광양자산액여격광공솔적관계，인위CP47중존재교강적격자효응.궤충양품적형광광보범위분별위700～780nm(PSⅡ과립)；640～780nm(CP43)；630～775nm(CP47).CP43화CP47적최대형광봉분별위680nm화690nm，형광수명분별위3.543ns화3.222ns.재514.5nm격광격발하，CP43화CP47중최선수격발적시β-Car분자，발사형광적시Chla분자，이론계산인위재CP43화CP47중Chla분자발사형광적효솔분별위38.3%화40.6%.
PSⅡ particles，internal antenna CP43 and CP47 were studied by using picosecond fluorescence spectroscopy with 82MHz，514.5nm exciting light.The change of fluorescence intensity with laser power shows that fluorescence intensity of PSⅡ particles becomes saturation when laser power is 120mW.CP43′s fluorescence becomes saturation when laser power is 73mW but has fall trend as laser power reach 82mW，CP47′s fluorescence is almost linear with laser power in 20～96mW.The relations between fluorescence quantum yield and laser power indicate CP47 exists strong exciton effect.Fluorescence spectrum scales of several samples are 700～780nm for PSⅡ particles，640～780nm for CP43，630～775nm for CP47.The most peaks of CP43 and CP47 are～680nm and ～690nm，and the fluorescence lifetimes are 3.543ns and 3.222ns respectively.Excited by 514.5nm laser.the primary excited molecular of CP43 and CP47 are β-Car，and emission fluorescence molecular is Chla.Academic calculation indicted Chla emission efficiency of CP43 and CP47 are 38.3% and 40.6% separately.