CAJ | 학술논문

为适应固氮酶蛋白等厌氧蛋白质空间晶体生长的要求，应在地面用简易而适用的厌氧加样装置以优化这类蛋白的结晶条件。用塑料袋或简易箱代替固氮酶实验室常用的笨重厌氧箱，获得了缺失nifZ固氮菌(Azotobactervinelandii Lipmann)突变种的MoFe蛋白和含锰固氮培养基中生长的UW3的MnFe蛋白晶体。并在远离固氮酶实验室的地方，使用由小食品塑料袋和急救用的氩气袋组成的更轻便的厌氧装置，用坐滴法也能使这两种蛋白结晶出来。结果表明，利用上述简易厌氧装置有望达到以上2种厌氧蛋白空间晶体生长的要求。
위괄응고담매단백등염양단백질공간정체생장적요구，응재지면용간역이괄용적염양가양장치이우화저류단백적결정조건。용소료대혹간역상대체고담매실험실상용적분중염양상，획득료결실nifZ고담균(Azotobactervinelandii Lipmann)돌변충적MoFe단백화함맹고담배양기중생장적UW3적MnFe단백정체。병재원리고담매실험실적지방，사용유소식품소료대화급구용적아기대조성적경경편적염양장치，용좌적법야능사저량충단백결정출래。결과표명，이용상술간역염양장치유망체도이상2충염양단백공간정체생장적요구。
In order to meet the requirement for crystalline growth ofO2-susceptible proteins in space, crystallization conditions on the earth was optimized for the proteins using a simple and suitable device for anaerobic addition of the protein samples. Nitrogenase is susceptible to O2. ΔnifZ MoFe protein from a nifZ deleted strain and MnFe protein from mutant strain UW3 grown on a medium containing Mn were crystallized at the first time in the world using an anaerobic device equipped with plastic bags or using a small simplified box, as a replacement for the cumbersome dry box. And the proteins could be also crystallized far from laboratory by sitting-drop method using a much lighter device. It was equipped with a smaller plastic food bag and a first-aid bag filled with Ar, as a substitute for the cumbersome dry box and the Ar cylinder, respectively. The results showed that the device could meet the requirement for studies on crystal growth of the above anaerobic proteins in space.