CAJ | 학술논문

应用60Co γ射线对侧耳属变异株的双核菌丝体进行辐射诱变处理，并对其再生菌落进行菌丝形态学、细胞学、生理生化鉴定。结果发现，诱变株菌落形态发生明显变化，气生菌丝减少，生长过程减慢，细胞直径变大，分枝增多，与出发菌株拮抗线明显，EST，POD同工酶显示其酶谱差异显著。结实性研究表明，诱变株较出发株子实体分化正常，产量提高，抗性增强，品质良好。显示60 Co γ射线辐射诱变是改良侧耳属菌株的有效途径。
응용60Co γ사선대측이속변이주적쌍핵균사체진행복사유변처리，병대기재생균락진행균사형태학、세포학、생리생화감정。결과발현，유변주균락형태발생명현변화，기생균사감소，생장과정감만，세포직경변대，분지증다，여출발균주길항선명현，EST，POD동공매현시기매보차이현저。결실성연구표명，유변주교출발주자실체분화정상，산량제고，항성증강，품질량호。현시60 Co γ사선복사유변시개량측이속균주적유효도경。
The dicaryotic hyphae of the variant strain of P.ostreatus was treated by radiation of 60　Co γ ray. Mycelial morpholagical,cytological and physiological-biochemical characteristics of its mutant strain were studied.The results showed that the mutant strain had less aerial mycelium.Lower colony growth rate,bigger mycelium diameter,more branch on vegetative growth phase and had more fruitbody formation,higher yield,better quality on reproductive growth phase than the initial strain.Clear ant-agnistic line and significant differences of isoenzyme pattern of EST and POD existed between the mutant and initial strains.It showed that induced mutation from dicaryotic hyphae by 60　Co γ ray was an effective means of gaining excellent rejuvenation strain of P.ostreatus.