CAJ | 학술논문

目的采用OLETF大鼠来观察血脂变化对2型糖尿病鼠肾小球细胞外基质含量的影响。方法实验动物分为三组即正常对照组、糖尿病组和非诺贝特治疗的糖尿病组。非诺贝特20mg*kg-1*d-1灌胃22周。用免疫组化的方法检测肾小球细胞外基质成分含量的变化。结果糖尿病组血清总胆固醇、甘油三酯、极低密度脂蛋白和高密度脂蛋白水平明显高于正常对照 (P＜0.05)，同时细胞外基质成分(Ⅳ型胶原、层粘连蛋白、纤维连接蛋白)含量亦较正常对照组增加(三者P＜0.01)，降脂治疗后肾脏细胞外基质积聚明显减少(与糖尿病未治疗组比P＜0.05)。结论高脂血症可能与糖尿病肾小球硬化的发生有关，降脂治疗能够减缓肾脏的损害从而起到保护肾脏的作用。
목적채용OLETF대서래관찰혈지변화대2형당뇨병서신소구세포외기질함량적영향。방법실험동물분위삼조즉정상대조조、당뇨병조화비낙패특치료적당뇨병조。비낙패특20mg*kg-1*d-1관위22주。용면역조화적방법검측신소구세포외기질성분함량적변화。결과당뇨병조혈청총담고순、감유삼지、겁저밀도지단백화고밀도지단백수평명현고우정상대조 (P＜0.05)，동시세포외기질성분(Ⅳ형효원、층점련단백、섬유련접단백)함량역교정상대조조증가(삼자P＜0.01)，강지치료후신장세포외기질적취명현감소(여당뇨병미치료조비P＜0.05)。결론고지혈증가능여당뇨병신소구경화적발생유관，강지치료능구감완신장적손해종이기도보호신장적작용。
Objective To investigate the effect of changes in plasma lipid levels on the accumulation of glomerular extracellular matrix in type 2 diabetic rats.Methods Rats were divided into three groups,namely,normal control,diabetes mellitus treated with Fenofibrate,which was gastrically administrated in the dose of 20mg·kg -1 ·d -1 for 22 weeks.A quantitative analysis of the components of glomerular extracellular matrix (ECM) were performed with immunoperoxide (ABC) method and the computer imagine analysis system.Results The result showed that diabetic rats had significantly higher plasma lipid levels and accumulation of ECM including collagen Ⅳ,laminine and fibronectin than the normal control ( P <0.01).22 weeks after the treatment with Fenofibrate,accumulation of ECM in diabetes treamtnent group was less marked as compared with the diabetes without treatment group ( P <0.05).Conclusion It is suggested that hyperlipidemia play a role in the pathogenesis of diabetic nephropathy.Lipid-lowering therapy may retard the progression of glomerulosclerosis in diabetes mellitus.