CAJ | 학술논문

测定三组Wistar大鼠分别灌胃给与受试制剂和两组参比制剂后不同时刻血浆中水飞蓟宾的浓度。采用色谱柱为KromasilC18柱(5μm粒径，200mm×4.6mmI.D.)，流动相为乙腈-甲醇-磷酸二氢胺缓冲液（体积比为24∶24∶50，pH=5），检测波长为UV288nm。0.25～20.00mg/L范围内线性关系良好(r=0.9976)，日内，日间RSD分别≤5.99%和≤6.91%。方法简便快速，适用于该药的动物生物利用度试验研究。
측정삼조Wistar대서분별관위급여수시제제화량조삼비제제후불동시각혈장중수비계빈적농도。채용색보주위KromasilC18주(5μm립경，200mm×4.6mmI.D.)，류동상위을정-갑순-린산이경알완충액（체적비위24∶24∶50，pH=5），검측파장위UV288nm。0.25～20.00mg/L범위내선성관계량호(r=0.9976)，일내，일간RSD분별≤5.99%화≤6.91%。방법간편쾌속，괄용우해약적동물생물이용도시험연구。
A RP-HPLC method was developed to determinate silybin in the ratplasma of three groups to which the test formulation and reference formulation were administered. An ODS column was used. The mobile phase consisted of acetonitrile, methanol and NH4H2PO4 buffer (24∶24∶50,V/V,pH=5). The UV detector was set at 288 nm. The liner calibration curves were obtained in the concentration range of 0.25(20 mg/L. The within-day and between-day RSD were within 5.99% and 6.91%, respectively. The method is fast, simple and suitable for the bioavailability of silybin.