CAJ | 학술논문

目的：消减文库构建过程中，用PCR技术快速筛选重组阳性克隆。方法：将白色单菌落加入氨苄抗性LB培养液中，37 ℃摇振培养过夜，取细菌悬液作PCR模板。结果和结论：以PCR方法筛查重组阳性克隆，可以简便快速鉴定重组阳性克隆，不需提取质粒。筛选重组阳性克隆可直接用细菌悬液作PCR模板，在消减文库构建时，能大大提高工作效率。
목적： 소감문고구건과정중，용PCR기술쾌속사선중조양성극륭。방법：장백색단균락가입안변항성LB배양액중，37 ℃요진배양과야，취세균현액작PCR모판。결과화결론：이PCR방법사사중조양성극륭，가이간편쾌속감정중조양성극륭，불수제취질립。사선중조양성극륭가직접용세균현액작PCR모판，재소감문고구건시，능대대제고공작효솔。
Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.Methods：The recombinant colonies were transferred into LB culture medium with ampicillin,and shaken overnight at 37℃，then took the medium for PCR templet.Results and Conclusion ：The PCR screening method was convenient and fast for confirming positive recombinant clone,and there was no need for preparation and purification of the plasmid.Recombinant clone can be analyzed directly with the use of PCR.It is very efficient,especially in establishment of subtractive library of cDNA.