CAJ | 학술논문

目的：探讨黄芪对造血功能的影响及作用机制。方法：采用造血祖细胞体外培养和体内扩散盒植入技术，观察黄芪对辐照小鼠粒-单系造血祖细胞(CFU-GM)的影响。结果：体内培养证明，辐照前、后用药组CFU-GM分别为(27.14±5.88)个/(4×105BMC)和(26.38±4.90)个/(4×105BMC)，均较辐照对照组(16.58±3.37)个/(4×105BMC)明显增高(P＜0.01)，而体外培养未显示刺激活性。结论：黄芪可刺激体内造血干细胞的增殖与分化，对辐照小鼠的骨髓具有保护作用。
목적：탐토황기대조혈공능적영향급작용궤제。방법：채용조혈조세포체외배양화체내확산합식입기술，관찰황기대복조소서립-단계조혈조세포(CFU-GM)적영향。결과：체내배양증명，복조전、후용약조CFU-GM분별위(27.14±5.88)개/(4×105BMC)화(26.38±4.90)개/(4×105BMC)，균교복조대조조(16.58±3.37)개/(4×105BMC)명현증고(P＜0.01)，이체외배양미현시자격활성。결론：황기가자격체내조혈간세포적증식여분화，대복조소서적골수구유보호작용。
Aim：To study the effects of Radix Astragali on hemopoiesis and its mechanism.Methods：By using cell culture of hemopoietic progenitors in vitro and diffusion chamber implantation in vivo,we observed the effects of Radix Astrogali on colony forming unit-granulocyte ／macrophage(CFU-GM).Results：In diffusion chambers in vivo,the number of CFU-GM were(27.14±5.88)/(4×105BMC) and(26.38±4.90)/(4×105BMC) in the Radix Astragali pretreated or treated groups,respectively,which were significantly higher than that of irradiated control group(P＜0.01)，but there was no stimulation activity in vitro on irradiated mice.Conclusion：Radix Astragali can stimulate the proliferation and differentiation of the CFU-GM in diffusion chambers in vivo and may protect bone marrow of irrdiated mice.