CAJ | 학술논문

白喉型鸡痘病毒Gibbs株能在鸡胚绒毛尿囊膜上良好增殖，接种后120小时，绒毛尿囊膜明显水肿，且有淡黄色痘斑，其毒价达到105.25～105.83EID50/0.2ml。用10倍稀释的病毒悬液肌肉注射13日龄雏鸡，无任何不良反应。用100倍稀释病毒悬液皮下刺种免疫14～40日龄鸡，接种后3～6天，接种局部出现痘痂。接种后14天，用10倍稀释的Gibbs株病毒液二次皮下刺种或用鸡痘野毒株经腿部毛囊涂擦，免疫鸡未见任何局部反应或其它异常，保护率达100%。免疫后165天，保护率仍达80%。试验结果证实该毒株具有良好的免疫原性和安全性。
백후형계두병독Gibbs주능재계배융모뇨낭막상량호증식，접충후120소시，융모뇨낭막명현수종，차유담황색두반，기독개체도105.25～105.83EID50/0.2ml。용10배희석적병독현액기육주사13일령추계，무임하불량반응。용100배희석병독현액피하자충면역14～40일령계，접충후3～6천，접충국부출현두가。접충후14천，용10배희석적Gibbs주병독액이차피하자충혹용계두야독주경퇴부모낭도찰，면역계미견임하국부반응혹기타이상，보호솔체100%。면역후165천，보호솔잉체80%。시험결과증실해독주구유량호적면역원성화안전성。
Gibbs strain,which is diphterin type fowl pox virus,may replicates well in 10-day-old embryonated chicken eggs via the dropped chorio allantoic membrane (CAM)route.120 hours post inoculation(pi),the swelling and pox reactions was observed in CAM.Moreover,the titer of virus suspersion is 105.25～105.83EID50 per 0.2ml.The minimal immune dose by the conventional wing-web route was 10-5 0.1ml.13-day-old chickens,inoculated by intramuscular injection with 1∶10 virus dilution,no abnormal signs was observed.Vaccinated chickens with 1∶100 dilution,developing lesion at site of inoculation.At 14 day post inoculation,these immuned chickens were challenged with a virulent fowl pox strain HL or Gibbs strain,which protective rate was 100 percent,provided excellent protection.The duration of immunity lasted 5.5 months.