CAJ | 학술논문

目的观察新城疫鸡瘟病毒及其HN基因与F基因对裸小鼠人肺腺癌移植瘤的抗肿瘤作用，并探讨其机制。方法建立人肺腺癌细胞系在裸小鼠体内移植瘤动物模型，两组实验组瘤体内分别一次局部注射NDV病毒及携带HN及F基因的质粒(pSV-HN及pSV-F)作抗肿瘤研究，同时设PBS对照。观察5周，绘制肿瘤生长曲线，处死后量瘤重，并作电镜和光镜检查，观察其病理学改变。结果 NDV及其基因的质粒(pSV-HN及pSV-F)对裸小鼠人肺腺癌移植瘤的生长有明显的抑制作用(抑瘤率分别为71.62％和79.40％)。NDV组及质粒组与对照组瘤重均值之间差异显著（P＜0.01）。对照组14％的肿瘤发生肝及肺的转移。NDV组电镜下可见肿瘤细胞内NDV粒子出芽。结论 NDV对裸小鼠人肺腺癌移植瘤有明显的抑瘤作用。pSV-HN及pSV-F亦明显的抑制裸小鼠人肺腺癌移植瘤生长，其抑瘤作用机制与NDV选择性地在肿瘤细胞内增殖并导致肿瘤细胞凋亡及溶解肿瘤细胞有关，由此推测在此过程中HN及F基因可能起主要作用。
목적관찰신성역계온병독급기HN기인여F기인대라소서인폐선암이식류적항종류작용，병탐토기궤제。방법건립인폐선암세포계재라소서체내이식류동물모형，량조실험조류체내분별일차국부주사NDV병독급휴대HN급F기인적질립(pSV-HN급pSV-F)작항종류연구，동시설PBS대조。관찰5주，회제종류생장곡선，처사후량류중，병작전경화광경검사，관찰기병이학개변。결과 NDV급기기인적질립(pSV-HN급pSV-F)대라소서인폐선암이식류적생장유명현적억제작용(억류솔분별위71.62％화79.40％)。NDV조급질립조여대조조류중균치지간차이현저（P＜0.01）。대조조14％적종류발생간급폐적전이。NDV조전경하가견종류세포내NDV입자출아。결론 NDV대라소서인폐선암이식류유명현적억류작용。pSV-HN급pSV-F역명현적억제라소서인폐선암이식류생장，기억류작용궤제여NDV선택성지재종류세포내증식병도치종류세포조망급용해종류세포유관，유차추측재차과정중HN급F기인가능기주요작용。
Objective　To evaluate the anti-tumor effects of NDV and two genes of virus(HN and F) on athymic mice with human adenocarcinoma xenografts, and to investigate the mechanisms of its oncolytic role. Methods　The experimental model of lung adenocarcinoma xenograft was established. The two experimental groups of athymic mice were given intratumoral injections of NDV and plasmids only once, and compared with PBS controls in the same time. Measure the volume of tumors for 5 weeks and make a curve of the volume. These mice were killed after 5 weeks, and the weight of the tumors was measured. The histological and ultrastructral changes were observed by electromicroscope and microscope. Results　After one injection of live NDV and plasmids, the tumor growth was significantly suppressed (The median inhibitory rate was 71.62％ and 79.40％ respectively). The median weight of tumor of mice treated with NDV was remarkably lower than that of mice treated with PBS, and that of the mice treated with plasmids (P＜0.01). 14％ of the control group had liver and lung metastasis of the tumor, but no metastasis was found in the experimental groups. A great quantity of NDV viron budding was found in the NDV group. Conclusion　NDV could replicate in human lung adenocarcinoma xenografts, leading directly to a potent anti-tumor effect after one injection of live NDV. During the oncolytic process, the gene HN and gene F may play an important role.