上海医科大学学报
上海醫科大學學報
상해의과대학학보
JOURNAL OF SHANGHAI MEDICAL UNIVERSITY
2000年
6期
453-456
,共4页
徐韶华%廖劲晖%于伟英%朱运松
徐韶華%廖勁暉%于偉英%硃運鬆
서소화%료경휘%우위영%주운송
尿激酶型纤溶酶原激活剂%尿激酶型纤溶酶原激活剂受体
尿激酶型纖溶酶原激活劑%尿激酶型纖溶酶原激活劑受體
뇨격매형섬용매원격활제%뇨격매형섬용매원격활제수체
urokinase plasminogen activator%urokinase plasminogen activator receptor
目的研究尿激酶型纤溶系统组分uPA、uPAR、tPA及PAI?1在人乳腺癌细胞侵袭中的作用。方法以3株具有不同侵袭转移能力的乳腺癌细胞株作为研究对象，应用RT?PCR方法比较纤溶组分在此3株细胞中的表达，牛奶板法检测细胞培养上清中的纤溶活性，用Boyden小室模型测定细胞侵袭能力。结果发现MDA?MB?231细胞表达较高水平的uPA、uPAR、PAI?1和中等水平的tPA，无血清培养上清中总纤溶活性和uPA纤溶活性最高；MDA?MB?435细胞表达较低水平的uPA和较高水平的tPA，但未测出uPAR和PAI?1的表达，无血清培养上清中总纤溶活性较高，主要是tPA活性；MCF?7细胞表达较低水平的uPAR和较高水平的PAI?1，但未测出uPA和tPA的表达，无血清培养上清中几乎没有纤溶活性。与纤溶活性相一致的是，Boyden小室模型实验结果发现MDA?MB?231在3株细胞中体外侵袭能力最强，MDA?MB?435次之，MCF?7则几乎无体外侵袭能力。经抗uPA和抗uPAR抗体预处理MDA?MB?231细胞，分别使侵袭能力下降83.1%和43.9%(P<0.05)。结论 uPA和uPAR的活性与乳腺癌细胞的侵袭转移能力密切相关
目的研究尿激酶型纖溶繫統組分uPA、uPAR、tPA及PAI?1在人乳腺癌細胞侵襲中的作用。方法以3株具有不同侵襲轉移能力的乳腺癌細胞株作為研究對象，應用RT?PCR方法比較纖溶組分在此3株細胞中的錶達，牛奶闆法檢測細胞培養上清中的纖溶活性，用Boyden小室模型測定細胞侵襲能力。結果髮現MDA?MB?231細胞錶達較高水平的uPA、uPAR、PAI?1和中等水平的tPA，無血清培養上清中總纖溶活性和uPA纖溶活性最高；MDA?MB?435細胞錶達較低水平的uPA和較高水平的tPA，但未測齣uPAR和PAI?1的錶達，無血清培養上清中總纖溶活性較高，主要是tPA活性；MCF?7細胞錶達較低水平的uPAR和較高水平的PAI?1，但未測齣uPA和tPA的錶達，無血清培養上清中幾乎沒有纖溶活性。與纖溶活性相一緻的是，Boyden小室模型實驗結果髮現MDA?MB?231在3株細胞中體外侵襲能力最彊，MDA?MB?435次之，MCF?7則幾乎無體外侵襲能力。經抗uPA和抗uPAR抗體預處理MDA?MB?231細胞，分彆使侵襲能力下降83.1%和43.9%(P<0.05)。結論 uPA和uPAR的活性與乳腺癌細胞的侵襲轉移能力密切相關
목적연구뇨격매형섬용계통조분uPA、uPAR、tPA급PAI?1재인유선암세포침습중적작용。방법이3주구유불동침습전이능력적유선암세포주작위연구대상，응용RT?PCR방법비교섬용조분재차3주세포중적표체，우내판법검측세포배양상청중적섬용활성，용Boyden소실모형측정세포침습능력。결과발현MDA?MB?231세포표체교고수평적uPA、uPAR、PAI?1화중등수평적tPA，무혈청배양상청중총섬용활성화uPA섬용활성최고；MDA?MB?435세포표체교저수평적uPA화교고수평적tPA，단미측출uPAR화PAI?1적표체，무혈청배양상청중총섬용활성교고，주요시tPA활성；MCF?7세포표체교저수평적uPAR화교고수평적PAI?1，단미측출uPA화tPA적표체，무혈청배양상청중궤호몰유섬용활성。여섬용활성상일치적시，Boyden소실모형실험결과발현MDA?MB?231재3주세포중체외침습능력최강，MDA?MB?435차지，MCF?7칙궤호무체외침습능력。경항uPA화항uPAR항체예처리MDA?MB?231세포，분별사침습능력하강83.1%화43.9%(P<0.05)。결론 uPA화uPAR적활성여유선암세포적침습전이능력밀절상관
Purpose To investigate the role of urokinase plasminogen activator (uPA),uPA receptor (uPAR),tissue type plasminogen activator (tPA) and plasminogen activator inhibitor 1 (PAI-1) in the invasiveness of human breast cancer cells. Methods Three human breast cancer cell lines with different invasive ability were taken as research targets.RT-PCR and milk plates methods were used to detect the expression of uPA system members and the PA activities,respectively.Modified Boyden's chamber model was employed to detect the invasive ability of cancer cell. Results MDA-MB-231 could express high level of uPA,uPAR,PAI-1 and low level of tPA.MDA-MB-435 could express lower level of uPA and hight level of tPA,but no PAI-1 and uPAR were detected.MCF-7 could express lower level of uPAR and high level of PAI-1,but no uPA and tPA were detected.MDA-MB-231 cells showed the highest total PA and uPA activity.MDA-MB-435 cells also showed high total PA activity,but almost all the activity owed to tPA.MCF-7 showed almost no PA activity.Correlated with their PA activities,MDA-MB-231 was found the most invasive in vitro,followed by MDA-MB-435,and MCF-7 almost had no invasive ability.The antibodies against uPA and uPAR were significantly effective in reducing the matrigel invasiveness of MDA-MB-231 by approximately 83.1% and 43.9% respectively (P<0.05). Conclusions Co-expression of uPA,uPAR and PAI-1 in human breast cancer highly correlates with the invasiveness in vitro.
