CAJ | 학술논문

目的：研究正常人外周血CD8＋T细胞体外活化表达早期活化标志CD69分子及γ-干扰素(IFN-γ)的规律.方法：以佛波醇酯(PDB)+离子霉素(Ion)或植物血凝素(PHA)体外活化人外周血单个核细胞(PBMC)，利用流式细胞术分析CD8＋T细胞表达CD69及IFN-γ的情况.结果：PDB＋Ion和PHA活化CD8+T细胞CD69的表达率(%)分别为78.4±5.3和27.4±4.4(±s)，明显高于对照组(1.5±0.6)(P＜0.05).当monensin存在时以PDB＋Ion及PHA刺激4h后IFN-γ阳性CD8＋T细胞的百分比分别为29.7±6.5及5.9±1.8，均明显高于对照组(0.7±0.2)(P＜0.05)，其中前者表达率高于后者(P＜0.05).结论：PDB＋Ion和PHA均可刺激CD8＋T细胞表达CD69以及IFN-γ，前者的刺激作用明显强于后者.
목적：연구정상인외주혈CD8＋T세포체외활화표체조기활화표지CD69분자급γ-간우소(IFN-γ)적규률.방법：이불파순지(PDB)+리자매소(Ion)혹식물혈응소(PHA)체외활화인외주혈단개핵세포(PBMC)，이용류식세포술분석CD8＋T세포표체CD69급IFN-γ적정황.결과：PDB＋Ion화PHA활화CD8+T세포CD69적표체솔(%)분별위78.4±5.3화27.4±4.4(±s)，명현고우대조조(1.5±0.6)(P＜0.05).당monensin존재시이PDB＋Ion급PHA자격4h후IFN-γ양성CD8＋T세포적백분비분별위29.7±6.5급5.9±1.8，균명현고우대조조(0.7±0.2)(P＜0.05)，기중전자표체솔고우후자(P＜0.05).결론：PDB＋Ion화PHA균가자격CD8＋T세포표체CD69이급IFN-γ，전자적자격작용명현강우후자.
Aim: To study the details of the expression of early activationantigen CD69 and interferon-γ (IFN-γ) by in vitro activated CD8＋ T cells from normal human peripheral blood. Methods: Flow cytometry was adopted to detect the expression of CD69 and IFN-γ by CD8＋ T cells in response to the stimulation of phorbol ester (PDB) plus ionomycin or phytohemagglutinin (PHA). Results: The expression percentages of CD8＋T cells in response to PDB plus ionomycin and PHA were 78.4±5.3 and 27.4±4.4(±s) respectively, which were significantly higher than that of control (1.5±0.6)(P＜0.05). In the presence of monensin, the ratio of IFN-γ positive CD8+T cells by PDB plus ionomycin or PHA stimulation for 4 hours increased from 0.7±0.2 in control group to 29.7±6.5 and 5.9±1.8 respectively (P＜0.05), with the former higher than the latter(P＜0.05). Conclusion: Both PDB plus ionomycin and PHA are able to activate the expression of CD69 and IFN-γ, and the effect of the former is stronger than that of the latter.