中国神经科学杂志
中國神經科學雜誌
중국신경과학잡지
CHINESE JOURNAL OF NEUROSCIENCE
2000年
2期
156-159
,共4页
NG-硝基-L-精氨酸甲酯%精氨酸%催产素%低血压%一氧化氮
NG-硝基-L-精氨痠甲酯%精氨痠%催產素%低血壓%一氧化氮
NG-초기-L-정안산갑지%정안산%최산소%저혈압%일양화담
NG-nitroarginine methyl ester%arginine%oxytocin%hypotension%nitric oxide
取戊巴比妥麻醉大鼠向侧脑室内分别注射一氧化氮(NO)合酶的底物L-精氨酸和NO合酶抑制剂NG-硝基-L-精氨酸甲酯(L-NAME),用放射免疫法测定血浆中催产素(OT)水平.结果:侧脑室内注射L-精氨酸(100 g/L,10 霯,n=8)和L-NAME(54.0 g/L,5 霯,n=12),对OT的基础分泌无明显影响;侧脑室内注射5 霯 L-NAME(剂量1:27.0 g/L,n=9;剂量2:54.0 g/L,n=8),可进一步增强静脉输注硝普钠引起低血压所诱导的OT分泌升高反应.结果表明L-NAME能加强低血压诱发的OT反射性释放作用,提示NO可能是OT反射性释放的抑制因子.
取戊巴比妥痳醉大鼠嚮側腦室內分彆註射一氧化氮(NO)閤酶的底物L-精氨痠和NO閤酶抑製劑NG-硝基-L-精氨痠甲酯(L-NAME),用放射免疫法測定血漿中催產素(OT)水平.結果:側腦室內註射L-精氨痠(100 g/L,10 霯,n=8)和L-NAME(54.0 g/L,5 霯,n=12),對OT的基礎分泌無明顯影響;側腦室內註射5 霯 L-NAME(劑量1:27.0 g/L,n=9;劑量2:54.0 g/L,n=8),可進一步增彊靜脈輸註硝普鈉引起低血壓所誘導的OT分泌升高反應.結果錶明L-NAME能加彊低血壓誘髮的OT反射性釋放作用,提示NO可能是OT反射性釋放的抑製因子.
취무파비타마취대서향측뇌실내분별주사일양화담(NO)합매적저물L-정안산화NO합매억제제NG-초기-L-정안산갑지(L-NAME),용방사면역법측정혈장중최산소(OT)수평.결과:측뇌실내주사L-정안산(100 g/L,10 등,n=8)화L-NAME(54.0 g/L,5 등,n=12),대OT적기출분비무명현영향;측뇌실내주사5 등 L-NAME(제량1:27.0 g/L,n=9;제량2:54.0 g/L,n=8),가진일보증강정맥수주초보납인기저혈압소유도적OT분비승고반응.결과표명L-NAME능가강저혈압유발적OT반사성석방작용,제시NO가능시OT반사성석방적억제인자.
To detect the effect of nitric oxide (NO)on basal and reflex release of oxytocin (OT),L-arginine(the substrate of NO synthase )and NG-nitro-L-arginine methyl ester (L-NAME,NO synthase inhibitor)were intracerebroventricularly(icv)administrated into the pentobarbital anesthetized rats and the plasma OT level was detected with radioimmunoassay.Injection (icv) of L-arginine(100 g/L,10 μL,n=8)and L-NAME (54.0 g/L,5 μL,n=12) had no significant effect on OT basal secretion.However,the elevated OT secretion in response to hypotension induced by intravenous infusion of sodium nitroprusside was dose-dependently augmented by icv injection of 5 μL L-NAME (dose 1:27.0 g/L,n=9；dose 2:54.0 g/L,n=8).The results indicate that L-NAME does not change basal OT secretion,but enhances OT reflex secretion evoked by hypotension,which suggests that NO may act as an inhibitory factor on reflex release of OT.
