东南大学学报(英文版)
東南大學學報(英文版)
동남대학학보(영문판)
JOURNAL OF SOUTHEAST UNIVERSITY
2000年
1期
106-110
,共5页
生物传感器%组织电极%修饰电极%二茂铁%L-酪氨酸
生物傳感器%組織電極%脩飾電極%二茂鐵%L-酪氨痠
생물전감기%조직전겁%수식전겁%이무철%L-락안산
biosensor%tissue-based membrane electrode%modified electrode%ferrocene%L-tyrosine
本文采用二茂铁作为电子传递介体,利用蘑菇组织含有丰富的酪氨酸酶,将蘑菇组织肉浆固定在二茂铁(PVC膜)修饰石墨电极上,用电流法测定L-酪氨酸浓度.研究了电极的制备和最佳实验条件,其线性响应范围为2.0×10-4~4.5×10-3 mol.L-1,响应时间小于5 min,电极寿命至少30?d,可用于实际样品分析.
本文採用二茂鐵作為電子傳遞介體,利用蘑菇組織含有豐富的酪氨痠酶,將蘑菇組織肉漿固定在二茂鐵(PVC膜)脩飾石墨電極上,用電流法測定L-酪氨痠濃度.研究瞭電極的製備和最佳實驗條件,其線性響應範圍為2.0×10-4~4.5×10-3 mol.L-1,響應時間小于5 min,電極壽命至少30?d,可用于實際樣品分析.
본문채용이무철작위전자전체개체,이용마고조직함유봉부적락안산매,장마고조직육장고정재이무철(PVC막)수식석묵전겁상,용전류법측정L-락안산농도.연구료전겁적제비화최가실험조건,기선성향응범위위2.0×10-4~4.5×10-3 mol.L-1,향응시간소우5 min,전겁수명지소30?d,가용우실제양품분석.
A new approach for assembling amperometric mushroom pulp tissue-based membrane electrode for determination of L-tyrosine analysis is proposed. Ferrocene is used as a mediator of electron transfer between tyrosinase in mushroom tissue and a graphite electrode. The optimal operation conditions are studied. The linear response range of the biosensor is 2.0×10-4to 4.5×10-3mol.L-1 with response time of less than 5 min and lifetime of at least 30 d. The biosensor can be applied to practical sample analysis.