CAJ | 학술논문

利用人工接毒方法对46份我国主要玉米自交系进行了两年抗甘蔗花叶病毒鉴定,筛选出高抗系8份(K22、CN962、P138、齐318、中自01、金黄96B、齐319、Pa405),抗病系7份(旱21、中自03、旱23、农大178、获白、K12、黄早四).用SSR标记研究了46份自交系的遗传多样性.49对引物共检测出168个等位基因变异,每对引物检测等位基因2～10个,平均为3.43个.UPGMA聚类分析表明,供试自交系可分为7群,划群结果与系谱和育种家经验基本相符.15份抗病毒自交系分散于4群(A、B、E、G),依据杂种优势原理,可用于改良同一群内感病系的抗性;其中群E和亚群GII被鉴定为抗病毒种质,可用于组建抗病群体.本文的研究结果为抗甘蔗花叶病毒玉米种质改良提供了重要信息.
이용인공접독방법대46빈아국주요옥미자교계진행료량년항감자화협병독감정,사선출고항계8빈(K22、CN962、P138、제318、중자01、금황96B、제319、Pa405),항병계7빈(한21、중자03、한23、농대178、획백、K12、황조사).용SSR표기연구료46빈자교계적유전다양성.49대인물공검측출168개등위기인변이,매대인물검측등위기인2～10개,평균위3.43개.UPGMA취류분석표명,공시자교계가분위7군,화군결과여계보화육충가경험기본상부.15빈항병독자교계분산우4군(A、B、E、G),의거잡충우세원리,가용우개량동일군내감병계적항성;기중군E화아군GII피감정위항병독충질,가용우조건항병군체.본문적연구결과위항감자화협병독옥미충질개량제공료중요신식.
Forty-six elite maize(Zea mays L.) inbred lines were evaluated for reaction to sugarcane mosaic virus under field conditions with artificial inoculation in 1999 and 2000. The results showed that 8 lines (K22, CN962, P138, Qi318, Zhongzi01, Jinhuang96B, Qi319, and Pa405)were highly resistant to SCMV,and 7(Han21, Zhongzi03, Han23, Nongda178, Huobai, K12,and Huangzao4)were resistant. The germplasm basis for resistance to SCMV in 46 inbreds was investigated by SSR markers. The average number of alleles per SSR locus was 3.43 with a range from 2 to 10. The UPGMA cluster analysis showed that the 46 inbreds could be classified into 7 distinct groups with several subgroups, which were generally consistent to their known pedigree information and breeder's experiences. Fifteen resistant inbreds were found in 4 groups (A,B,E,G), which could be used in germplasm improvement for SCMV resistance based on heterotic pattern, respectively. Group E and subgroup GII were identified as two important sources of resistance, which can be employed to synthesize the composites for SCMV resistance. The study provided useful information for germplasm improvement for resistance to SCMV.