CAJ | 학술논문

朱鹮是一种濒危鸟类.过度开发和栖息地的破坏导致了朱鹮日本种群的绝灭,而在中国目前也仅存一野生种群.本文利用NJ法和最大简约法分析了鹳形目鸟类977bp的线粒体DNA细胞色素b基因的部分序列,以期从DNA水平上阐明朱鹮的系统地位.结果表明,朱鹮与彩鹮和非洲琶鹭组成一姐妹群;NJ法分析得出的结果与DNA杂交分析结果一致,即((鹮科,鹭科),鹳科);而最大简约法分析的结果则与形态学结果一致,即((鹭科,鹳科),鹮科).同时,这两种系统树上较低的置信度,认为部分是由于可用细胞色素b基因序列的有限所致.
주환시일충빈위조류.과도개발화서식지적파배도치료주환일본충군적절멸,이재중국목전야부존일야생충군.본문이용NJ법화최대간약법분석료관형목조류977bp적선립체DNA세포색소b기인적부분서렬,이기종DNA수평상천명주환적계통지위.결과표명,주환여채환화비주파로조성일저매군;NJ법분석득출적결과여DNA잡교분석결과일치,즉((환과,로과),관과);이최대간약법분석적결과칙여형태학결과일치,즉((로과,관과),환과).동시,저량충계통수상교저적치신도,인위부분시유우가용세포색소b기인서렬적유한소치.
The Crested Ibis Nipponia nippon is one of the critically endangered species. Overexploitation and destruction ofhabitat led the Japanese population to extinction during the 20 th century. Only one wild population and two captive popula-tions are in China at present. Little is known about the molecular phylogeny of this species at the level of gene sequence, 977bpof the mitochondrial cytochrome-b gene was analyzed to clarify the phylogenetic status of N. nippon among the order Ciconi-iformes using NJ and parsimony analysis in the present study. Our results showed that N. nippon became a sister group to acluster of P. falcinellus and P. alba based on molecular analysis which were not congruent with the traditional classification.The branching pattern was ((Threskiornithidae, Ardeidae), Ciconiidae) in the NJ tree which was congruent with the phyloge-netic relationship inferred from DNA hybridization analysis. On the other hand, the brancing order in the parsimony tree was( (Ardeidae, Ciconiidae), Threskiornithidae) which was in agreement with the taxonomy based on the morphological analysis.Neither of the branching patterns in the trees was supported with high bootstrap values in our analysis. This is partly becausethe number of available cytochrome b gene sequences is limited for those families.