清华大学学报(英文版)
清華大學學報(英文版)
청화대학학보(영문판)
TSINGHUA SCIENCE AND TECHNOLOGY
2002年
6期
660-664
,共5页
张琳%周全%傅学奇%陈国强
張琳%週全%傅學奇%陳國彊
장림%주전%부학기%진국강
ceramide%sphingolipid%ergosterol%thin layer chromatography (TLC)%high-performance liquid chromatography (HPLC)%evaporative light scattering detection (ELSD)
Ceramides are important signaling molecules involved in a variety of cellular processes, including cell growth, differentiation, and apoptosis. Currently, different methods are used for ceramide analysis, some of which are insensitive or cumbersome. This paper described methods utilizing thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) followed by evaporative light scattering detection (ELSD) to detect ceramide directly in cell extracts without derivatization, which was proved to be efficient and reproducible. Five kinds of ceramides were used as standards. Both TLC and normal-phase HPLC/ELSD results indicate that yeast contains several kinds of ceramides.
