CAJ | 학술논문

使用装有液氦冷却的冷冻样品支架的300kV场发射冷冻电子显微镜拍摄了细胞质多角体病毒(CPV),对这些电镜照片的频谱分析显示图像分辨率优于0 6 nm.我们的三维重构结果使用了最新设计的图象管理和二十面体重构系统(IMIRS)软件包,这是第一个基于Microsoft Windows平台上运行的冷冻电镜三维重构软件包.从得到的一个初步的CPV三维结构,可以看出CPV是具有T=1的病毒壳体,它的核心半径为28 5nm,在二十面体的顶点有十二个具有五次对称的突起,使它的半径达到36nm.
사용장유액양냉각적냉동양품지가적300kV장발사냉동전자현미경박섭료세포질다각체병독(CPV),대저사전경조편적빈보분석현시도상분변솔우우0 6 nm.아문적삼유중구결과사용료최신설계적도상관리화이십면체중구계통(IMIRS)연건포,저시제일개기우Microsoft Windows평태상운행적냉동전경삼유중구연건포.종득도적일개초보적CPV삼유결구,가이간출CPV시구유T=1적병독각체,타적핵심반경위28 5nm,재이십면체적정점유십이개구유오차대칭적돌기,사타적반경체도36nm.
High resolution micrographs of cytoplasmic polyhedrosis virus (CPV) embedded in vitreous ice at liquid helium temperature were recorded in a 300 kV electron cryomicroscope (cryoEM) equipped with a field emission gun (FEG). Power spectrum analysis of these micrographs indicates that an image resolution better than 0 6 nm was obtained. We present our three dimensional (3D) reconstruction effort using a recently designed Image Management and Icosahedral ReconstructionSystem (IMIRS), which is the first software package that can carry out cryoEM 3D reconstruction on Microsoft Windows platform. A preliminary 3D reconstructionof the full CPV clearly reveals that the T=1 capsid shell of a radius of 28 5 nm encloses regularly spaced dsRNA layers and is decorated with twelve pentamericturrets extending to a radius of 36 0 nm on its icosahedral vertices.