植物病理学报
植物病理學報
식물병이학보
ACTA PHYTOPATHOLOGICA SINICA
2007年
6期
566-571
,共6页
刘勇%谢艳%廖白璐%Roy B.Mugiira%周雪平
劉勇%謝豔%廖白璐%Roy B.Mugiira%週雪平
류용%사염%료백로%Roy B.Mugiira%주설평
烟草曲叶病%双生病毒%云南省%烟草
煙草麯葉病%雙生病毒%雲南省%煙草
연초곡협병%쌍생병독%운남성%연초
tobacco leaf curl disease%geminiviruses%Yunnan province%Nicotiana tabacum
已报道云南省烟草曲叶病(Tobacco leaf curl disease,TLCD)由3种菜豆金色花叶病毒属(Begomovirus)的双生病毒引起.它们是烟草曲茎病毒(Tobacco curly shoot virus,TbCSV)、云南烟草曲叶病毒(Tobacco leaf curl Yunnan virus,TbLCYNV)和中国番茄黄化曲叶病毒(Tomato yellow leaf curl China virus,TYLCCNV).为了了解这3种病毒的分布,2000～2005年,从云南省4个市(州)采集了109个烟草曲叶病样品,利用3种病毒的特异引物对采集样品进行了PCR检测.从保山市的烟草曲叶病样品中检测到3种病毒,并且存在TbCSV+TbLCYNV、TbCSV+TYLCCNV和TbCSV+TbLYNV+TYLCCNV的复合侵染,从大理州、文山州和红河州的烟草曲叶病样品中仅检测到TYLCCNV.对39个分离物部分序列的系统进化分析结果表明,病毒分离物依据病毒种类和地理位置聚成几簇.对烟草曲叶病的流行和病害控制策略进行了讨论.
已報道雲南省煙草麯葉病(Tobacco leaf curl disease,TLCD)由3種菜豆金色花葉病毒屬(Begomovirus)的雙生病毒引起.它們是煙草麯莖病毒(Tobacco curly shoot virus,TbCSV)、雲南煙草麯葉病毒(Tobacco leaf curl Yunnan virus,TbLCYNV)和中國番茄黃化麯葉病毒(Tomato yellow leaf curl China virus,TYLCCNV).為瞭瞭解這3種病毒的分佈,2000～2005年,從雲南省4箇市(州)採集瞭109箇煙草麯葉病樣品,利用3種病毒的特異引物對採集樣品進行瞭PCR檢測.從保山市的煙草麯葉病樣品中檢測到3種病毒,併且存在TbCSV+TbLCYNV、TbCSV+TYLCCNV和TbCSV+TbLYNV+TYLCCNV的複閤侵染,從大理州、文山州和紅河州的煙草麯葉病樣品中僅檢測到TYLCCNV.對39箇分離物部分序列的繫統進化分析結果錶明,病毒分離物依據病毒種類和地理位置聚成幾簇.對煙草麯葉病的流行和病害控製策略進行瞭討論.
이보도운남성연초곡협병(Tobacco leaf curl disease,TLCD)유3충채두금색화협병독속(Begomovirus)적쌍생병독인기.타문시연초곡경병독(Tobacco curly shoot virus,TbCSV)、운남연초곡협병독(Tobacco leaf curl Yunnan virus,TbLCYNV)화중국번가황화곡협병독(Tomato yellow leaf curl China virus,TYLCCNV).위료료해저3충병독적분포,2000～2005년,종운남성4개시(주)채집료109개연초곡협병양품,이용3충병독적특이인물대채집양품진행료PCR검측.종보산시적연초곡협병양품중검측도3충병독,병차존재TbCSV+TbLCYNV、TbCSV+TYLCCNV화TbCSV+TbLYNV+TYLCCNV적복합침염,종대리주、문산주화홍하주적연초곡협병양품중부검측도TYLCCNV.대39개분리물부분서렬적계통진화분석결과표명,병독분리물의거병독충류화지리위치취성궤족.대연초곡협병적류행화병해공제책략진행료토론.
Three distinct geminivirus species Tobacco curly shoot virus (TbCSV), Tobacco leaf curl Yunnan virus (TbLCYNV) and Tomato yellow leaf curl China virus (TYLCCNV) belonging to the genus Begomovirus have previously been found associated with tobacco leaf curl disease (TLCD) in Yunnan province, China.For determination of their distribution, 109 samples were collected from symptom expressing tobacco plants in fields located in four districts of Yunnan province in the period from 2000 to 2005. Primer pairs specific to each of the three viruses were used to determine their occurrence. PCR detections revealed that all the three viruses occurred in Baoshan district while only TYLCCNV was found in the other three districts (Dali, Wenshan and Honghe ). Mixed infections comprising TbCSV + TbLCYNV, TbCSV + TYLCCNV and TbCSV +TbLYNV + TYLCCNV were found in Baoshan district. Comparisons and phylogenetic analysis of the partial sequences obtained from a total of 39 isolates showed that they clustered into different groups on the basis of virus species and geographical location. The epidemiology of TLCD and the development of strategies for effective disease management were discussed.
