中药新药与临床药理
中藥新藥與臨床藥理
중약신약여림상약리
TRADITIONAL CHINESE DRUG RESEARCH&CLINICAL PHARMACOLOGY
2009年
4期
363-366
,共4页
檀桂胃宁颗粒%甘草酸铵%含量测定%TLC法%HPLC法
檀桂胃寧顆粒%甘草痠銨%含量測定%TLC法%HPLC法
단계위저과립%감초산안%함량측정%TLC법%HPLC법
Tangui Weining granules%Glycyrrhizin%Determination%TLC%HPLC
目的 建立檀桂胃宁颗粒的质量控制方法.方法 采用TLC法对方中檀香和肉桂进行定性鉴别;采用HPLC法对处方中甘草酸的含量进行定量检测,色谱条件:色谱柱:Diamonsil C18(4.6 mm× 250 mm,5μm);流动相:甲醇-0.2 mol/L醋酸铵溶液-冰醋酸(60:40:1);流速:1.0 mL·min-1;检测波长:250 nm.结果 薄层色谱鉴别方法专属性强,重现性好,阴性对照无干扰;含量测定检查中甘草酸铵其他组分分离良好,线性范围为0.1568～1.568μg,r=0.999 8,平均回收率99.69%,RSD为0.58%.结论 薄层色谱方法简便快捷,结果可靠;含量测定方法操作简便、灵敏度高、准确,可用于檀桂胃宁颗粒中甘草酸铵的测定.
目的 建立檀桂胃寧顆粒的質量控製方法.方法 採用TLC法對方中檀香和肉桂進行定性鑒彆;採用HPLC法對處方中甘草痠的含量進行定量檢測,色譜條件:色譜柱:Diamonsil C18(4.6 mm× 250 mm,5μm);流動相:甲醇-0.2 mol/L醋痠銨溶液-冰醋痠(60:40:1);流速:1.0 mL·min-1;檢測波長:250 nm.結果 薄層色譜鑒彆方法專屬性彊,重現性好,陰性對照無榦擾;含量測定檢查中甘草痠銨其他組分分離良好,線性範圍為0.1568～1.568μg,r=0.999 8,平均迴收率99.69%,RSD為0.58%.結論 薄層色譜方法簡便快捷,結果可靠;含量測定方法操作簡便、靈敏度高、準確,可用于檀桂胃寧顆粒中甘草痠銨的測定.
목적 건립단계위저과립적질량공제방법.방법 채용TLC법대방중단향화육계진행정성감별;채용HPLC법대처방중감초산적함량진행정량검측,색보조건:색보주:Diamonsil C18(4.6 mm× 250 mm,5μm);류동상:갑순-0.2 mol/L작산안용액-빙작산(60:40:1);류속:1.0 mL·min-1;검측파장:250 nm.결과 박층색보감별방법전속성강,중현성호,음성대조무간우;함량측정검사중감초산안기타조분분리량호,선성범위위0.1568～1.568μg,r=0.999 8,평균회수솔99.69%,RSD위0.58%.결론 박층색보방법간편쾌첩,결과가고;함량측정방법조작간편、령민도고、준학,가용우단계위저과립중감초산안적측정.
Objective To establish a method for quality control of Tangui Weining granules. Methods TLC was used for the identification of Lignum Santali Albi and Cortex Cinnamomi. HPLC was used for the determination of Glycyrrhizin. The chromatographic conditions were as follows: Diamonsil C18(4. 6 mm ×250 mm, 5 μm)was used as a stationary phase, the mobile phase consisted of methanol-O. 2 mol/L ammonium acetate solution-acetic acid (60 : 40 : 1), the ducible, and the negative controll did not show interference. Glycyrrhizin was separated completely by HPLC and showed a good linearity in the range of 0. 1568 to 1. 568μg, r=0. 999 8, the average recovery was 99.69 %, and RSD was 0. 58 %. Conclusion TLC is simple, convenient and accurate. The method of HPLC is proved to be simple and accu-rate with a good reproducibility and can be used for the inspection of Glycyrrhizin in Tangui Weining Granules. This study provides a foundation for quality control of Tangui Weining granules.
