CAJ | 학술논문

[目的] 为了研究X染色体短串联重复序列(X-STR)基因座的遗传多态性,我们检测了DXS6803、DXS981、DXS6809、DXS6789和DXS7132 5个基因座在广东汉族人群中的多态性. [方法] 利用荧光标记引物PCR技术复合扩增上述5个基因座,并用ABI PRISM 3100毛细管电泳及GeneMapper ID3.1软件进行基因分型.[结果] 在363个广东汉族无关个体(男性:181个,女性:182个)中5个基因座共检出54个等位基因.多态性信息含量为0.6935 ～ 0.8177;女性个体识别率为0.8976 ～ 0.9562.三联体非父排除率为0.7805 ～ 0.8467.[结论] 这5个基因座多态性较高,在个体识别和亲权鉴定中具有重要的应用价值.
[목적] 위료연구X염색체단천련중복서렬(X-STR)기인좌적유전다태성,아문검측료DXS6803、DXS981、DXS6809、DXS6789화DXS7132 5개기인좌재엄동한족인군중적다태성. [방법] 이용형광표기인물PCR기술복합확증상술5개기인좌,병용ABI PRISM 3100모세관전영급GeneMapper ID3.1연건진행기인분형.[결과] 재363개엄동한족무관개체(남성:181개,녀성:182개)중5개기인좌공검출54개등위기인.다태성신식함량위0.6935 ～ 0.8177;녀성개체식별솔위0.8976 ～ 0.9562.삼련체비부배제솔위0.7805 ～ 0.8467.[결론] 저5개기인좌다태성교고,재개체식별화친권감정중구유중요적응용개치.
[Objective] To learn about the genetic diversity,we studied the five X-chromosomal STR (X-STR) loci in Guangdong Han Nationality Groups.[Methods] The five Loci (DXS6803,DXS981,DXS6809,DXS6789,and DXS7132) were amplified in a pentaplex PCR reaction.PCR products were analyzed using capillary electrophoresis and ABI prism 3100 Genetic Analyzer,with GeneMapper ID 3.1 Analysis Software.[Results] A total of 363 individuals (181 unrelated male and 182 unrelated female) from Guangdong Han population were tested,54 alleles were observed for these loci.Polymorphism information content is 0.6935 ～ 0.8177.Power of discrimination in females was 0.8976 ～ 0.9562.Mean exclusion chance for X-STR in standard trios with daughters was 0.7805 ～ 0.8467.[Conclusion] The five loci in the multiplex system provide high polymorphism information for forensic identification and paternity testing,particularly for difficult paternity deficiency cases.